Protein Nitration Analysis
Post-translational modifications (PTMs) are key mechanisms that regulate cellular function and signal transduction pathways. Protein nitration is a prevalent oxidative modification, typically occurring on tyrosine residues to form 3-nitrotyrosine. This process is mediated by reactive nitrogen species, such as peroxynitrite, particularly under conditions of oxidative stress. Nitration can induce significant changes in protein structure and function, thereby influencing cellular signaling, gene expression, protein-protein interactions, and metabolic processes. Recent studies have revealed that protein nitration plays a crucial role in the pathophysiology of various diseases, including cardiovascular disorders, neurodegenerative conditions, inflammatory responses, and cancer, where it has emerged as a potential biomarker. As such, precise analysis of protein nitration is indispensable for understanding its involvement in disease mechanisms and cellular regulation.
Radi. R. et al. Acc Chem Res. 2013.
Figure 1. Tyrosine, Tyrosyl Radical and 3-Nitrotyrosine
Services at MtoZ Biolabs
1. Targeted Protein Nitration Analysis
MtoZ Biolabs provides Targeted Protein Nitration Analysis that integrates immunoenrichment techniques with high-resolution LC-MS/MS to accurately identify and quantify nitration sites on specific proteins supplied by the client. This targeted approach enables detailed investigation of how protein nitration regulates signaling pathways, oxidative stress responses, and cellular functions. With optimized enrichment and detection workflows, the service ensures high sensitivity and reproducibility, supporting in-depth studies of protein-level regulation under physiological or pathological conditions.
2. Nitration Proteomics
For broader analyses, Nitration Proteomics applies a high-throughput LC-MS/MS-based workflow to comprehensively profile nitration modifications across the proteome. This approach provides a global view of nitration dynamics in diverse biological samples, uncovering modification patterns linked to metabolic regulation, oxidative stress, and disease mechanisms. The service delivers robust and quantitative datasets that facilitate biomarker discovery and systems-level understanding of protein nitration in health and disease.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform: MtoZ Biolabs established an advanced protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. Comprehensive Modification Site Mapping: Our methods allow the simultaneous identification of multiple nitration sites, resulting in a complete and detailed modification profile.
4. Versatile Quantitative Approaches: We offer both TMT/iTRAQ labeling and Label-Free methodologies, providing flexibility to accommodate diverse experimental requirements.
5. Efficient and Timely Results: Leveraging streamlined workflows and cutting-edge instrumentation, we ensure the rapid analysis of large sample volumes, delivering reliable, high-quality results within a short turnaround time.
Applications
Our protein nitration analysis services are highly applicable to the investigation of various diseases, including cardiovascular disorders, neurodegenerative diseases, inflammatory conditions, immune regulation, cancer, and diseases associated with oxidative stress. By precisely identifying nitration sites and performing detailed quantitative analyses, this service aids researchers in uncovering the pivotal roles of nitration in disease initiation, progression, and regulatory mechanisms, thereby supporting biomarker discovery and drug target screening.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs is dedicated to providing researchers worldwide with high-quality, accurate, and reliable PTM analysis services. With our cutting-edge technological platforms and extensive expertise, we deliver comprehensive data support to advance scientific research and foster innovation. Free project evaluation, welcome to learn more details!
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
