Protein Butyrylation Analysis

Lysine butyrylation (Kbu), an emerging PTM, occurs through the covalent attachment of a butyryl group to lysine residues in proteins. This modification is crucial for regulating gene expression, metabolism, epigenetics, and cellular differentiation. Investigating lysine butyrylation can deepen our understanding of its roles in metabolic regulation, gene expression, and disease progression, potentially offering new therapeutic opportunities for metabolic disorders and cancer.

MtoZ Biolabs provides comprehensive protein butyrylation analysis solutions that integrate optimized enrichment workflows with high-resolution LC MS MS, supporting both targeted protein studies and large-scale butyrylation proteomics. Our platform delivers accurate, reproducible, and biologically meaningful results to empower basic research and translational applications.

Services at MtoZ Biolabs

1. Target Protein Butyrylation Analysis

MtoZ Biolabs provides Target Protein Butyrylation Analysis to accurately identify and quantify butyrylation modifications on specific proteins designated by the client. This service integrates immunoprecipitation (Co-IP) with high-resolution LC-MS/MS to achieve targeted detection of butyrylated proteins or peptides. Using anti-butyryl–lysine–specific antibodies, modified proteins or peptides are efficiently enriched for precise localization of modification sites and quantitative evaluation of modification levels.

This targeted approach supports the investigation of site-specific butyrylation on proteins of interest, offering reliable experimental data for mechanistic research and functional validation.

2. Butyrylation Proteomics Analysis

MtoZ Biolabs offers Butyrylation Proteomics Analysis to comprehensively profile and quantify butyrylation modifications at the proteome level. Based on advanced high-sensitivity mass spectrometry platforms and specific anti-butyryl–lysine antibody enrichment, this service enables large-scale identification of butyrylated proteins and modification sites across diverse biological samples.

Through optimized sample preparation and bioinformatics analysis, MtoZ Biolabs reveals the distribution characteristics, functional classifications, and regulatory pathways associated with protein butyrylation, providing clients with complete, data-driven insights from modification discovery to functional pathway exploration.

If you are interested in our Protein Butyrylation Analysis, please feel free to contact us. We are dedicated to providing you with the best service.

Analysis Workflow

1. Sample Preparation: Proteins are extracted from cells or tissues and lysed using a buffer containing protease and deacetylase inhibitors to preserve the butyrylation modifications.

2. Protein Digestion: Trypsin is used to digest the proteins into peptides for subsequent mass spectrometry analysis.

3. Butyrylated Peptide Enrichment: Specific anti-butyrylation antibodies are used to enrich butyrylated peptides, minimizing interference from non-modified peptides.

4. Mass Spectrometry Analysis (LC-MS/MS): Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) is employed to detect and quantify the enriched butyrylated peptides and identify their modification sites.

5. Data Analysis: The mass spectrometry data are analyzed using appropriate software to determine the precise locations and abundance of butyrylation modifications.

Service Advantages

1. Advance Analysis Platform: Mtoz Biolabs established an advanced protein butyrylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time Charge: Our pricing is transparent, with no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all histone asymmetric dimethylation analysis data providing clients with acomprehensive data report.

4. Comprehensive Analysis: Both target protein butyrylation analysis and butyrylation proteomics can be conducted in MtoZ Biolabs.

Sample Submission Requirements

1. If you are providing tissue samples, please ship them to us on dry ice.

2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.

3. Sample transportation: Please ensure that samples are shipped with sufficient dry ice and choose the fastest possible shipping way to minimize the risk of sample degradation during transit.

4. Before proceeding with the formal experiment, we will test the samples you provide, and the formal analysis will begin once the samples pass the quality check.