Protein ADP-Ribosylation Identification Service

Mass spectrometry-based protein ADP-ribosylation identification provides a powerful approach to investigate post-translational modifications, offering precise site-level insights into ADP-ribosylation (ADPr) types and distributions. ADP-ribosylation is a dynamic and reversible modification in which ADP-ribose molecules are covalently attached to target proteins, regulating their structure, activity, localization, and interactions. It can occur as mono- or poly-ADPr on amino acid residues and plays essential roles in DNA repair, gene regulation, stress response, signal transduction, and apoptosis. Abnormal ADPr is linked to cancer and other diseases, and it also underlies the action of bacterial toxins such as cholera toxin and diphtheria toxin.

 

 

Mueller-Dieckmann, C. et al. Proc. Natl. Acad. Sci. 2006.

Figure 1. Protein ADP-ribosylation

MtoZ Biolabs provides high-quality Protein ADP-Ribosylation Identification Service using Thermo Fisher Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers combined with Nano-LC systems. With established data workflows and targeted enrichment methods, we deliver precise identification of ADPr sites, supporting systematic studies in both physiology and pathology, and enabling applications in basic research, disease mechanisms, and drug development.

  

Analysis Workflow

• Sample Preparation: Pretreatment to maintain ADPr stability.
• Enzymatic Digestion: Protease digestion to generate peptides for MS.
• ADP-ribosylated Peptides Enrichment: Antibody-based or chemical capture methods to enrich modified peptides.
• Mass Spectrometry Detection: Nano-LC with high-resolution LC-MS/MS for site and type identification.
• Data Analysis and Reporting: Database search and bioinformatics to confirm sites with comprehensive reports.

 



  

Why Choose MtoZ Biolabs?

✅ High-resolution Analysis: Orbitrap-based mass spectrometry combined with Nano-LC delivers exceptional sensitivity and accuracy for ADP-ribosylation identification.

✅ Expert Team: Our specialists bring extensive experience in ADPr research and provide tailored technical support to meet specific project goals.

✅ Fast Turnaround: We ensure timely delivery of high-quality results, helping you accelerate your research progress.

✅ Flexible Solutions: A range of service packages is available to accommodate diverse experimental requirements.

✅ Transparent Pricing: One-time charge model with clear, upfront costs—no hidden fees.

 

Sample Submission Suggestions

• Types: Cell lysates, tissue extracts, purified proteins, recombinant proteins, serum, plasma.
• Amount: ≥50 μg with ≥80% purity for target proteins; ≥1 mg with ≥1 μg/μL for mixtures.
• Storage: Store at –80°C, ship on dry ice, avoid freeze-thaw cycles.

Note: Please provide detailed information, including concentration, volume, preparation time, source, and group classification. For comprehensive submission guidance, contact the MtoZ Biolabs technical team.

  

Applications

• Protein Function Studies: Identify ADPr sites to understand pathway regulation.
• Biomarker Discovery: Detect disease-related ADPr patterns for discovery and validation.
• Drug Target Validation: Study ADPr effects on activity and interactions to accelerate drug development.
• Evolutionary and Microbiology Studies: Investigate ADPr conservation across species and microbial toxin mechanisms.

  

What Could Be Included in the Report?

• Comprehensive Experimental Details
• Materials, Instruments, and Methods
• High-quality Spectra and Visualized Charts
• Site-specific ADP-Ribosylation Identification with Detailed Annotation
• Bioinformatics Analysis
• Raw Data Files

  

MtoZ Biolabs delivers a professional Protein ADP-Ribosylation Identification Service with advanced mass spectrometry platforms and extensive expertise. We help researchers and biopharma companies analyze protein modification mechanisms with precision, supporting disease research, biomarker discovery, and therapeutic development. 

  

In addition, we provide identification services for phosphorylation, acetylation, methylation, and ubiquitination, enabling comprehensive protein modification profiling. Contact us today to start your PTM research journey.