Protein Acetylation Identification Service
- Sample Preparation: Careful pretreatment of protein samples to preserve modification integrity.
- Enzymatic Digestion: Application of tailored digestion protocols to generate detectable peptides.
- Separation and Detection: High-resolution LC-MS/MS combined with liquid chromatography for accurate analysis.
- Data Analysis: Database search and software interpretation to confirm acetylation sites with detailed annotation.
- Result interpretation: Functional annotation and pathway mapping to deliver a complete acetylation profile.
- Protein Function Research: Identify acetylation sites to uncover regulatory roles in signaling and functional modules.
- Biomarker Discovery: Characterize disease-specific acetylation patterns to support early diagnostics and personalized medicine.
- Drug Target Validation: Evaluate the impact of acetylation on protein activity and interactions to accelerate drug discovery pipelines.
- Plant and Agricultural Studies: Explore acetylation in plant growth, adaptation, and stress responses.
- Evolutionary Biology: Investigate conservation and diversity of acetylation across species to advance evolutionary insights.
- Comprehensive Experimental Details
- Materials, Instruments, and Methods
- High-quality Spectra and Visualized charts
- Site-specific Acetylation Identification with Detailed Annotation
- Bioinformatics Analysis
- Raw Data Files
Protein acetylation identification based on mass spectrometry is a powerful tool for investigating protein post-translational modifications. This approach enables precise site-specific detection of acetylation, one of the most common and significant PTMs. By adding acetyl groups to amino acid residues, proteins undergo changes in charge and spatial conformation, which influence their stability, localization, and molecular interactions. Acetylation plays essential roles in gene regulation, cell cycle progression, signal transduction, and metabolism, and it is closely linked to pathological processes, including cancer, metabolic disorders, and neurodegenerative diseases. Accurate acetylation profiling not only reveals dynamic regulatory mechanisms in cells but also provides valuable insights for biomarker discovery and novel drug target development.
Nallamilli, B. R. R. et al. PloS one, 2014.
Figure 1. Multiple Regulatory Functions of Protein Acetylation
Services of MtoZ Biolabs
MtoZ Biolabs offers high-quality protein acetylation identification service powered by advanced Thermo Fisher Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers combined with Nano-LC systems. Supported by robust data processing workflows, we deliver precise site identification and functional interpretation, helping researchers systematically explore the roles of acetylation in both physiological and pathological contexts.
Analysis Workflow
Figure 2. The Workflow of Protein Acetylation Identification
Why Choose MtoZ Biolabs?
✔ Advanced Analysis Platform: High-end Orbitrap-based systems with Nano-LC deliver sensitivity and precision.
✔ High-Data-Quality: Deep data coverage with strict data quality control. An AI-powered bioinformatics platform integrates all protein analysis data, providing clients with a comprehensive data report.
✔ Proven Expertise: Extensive experience in acetylation studies allows us to design customized strategies and provide professional support.
✔ Efficient Turnaround: Reliable results delivered within shortened timelines to accelerate your research progress.
✔ One-Time-Charge: Our pricing is transparent, with no hidden fees or additional costs.
Sample Submission Suggestions
Samples should be stored under low-temperature conditions and avoid repeated freeze-thaw cycles. Detailed submission guidelines are available upon request from MtoZ Biolabs.
Applications
What Could Be Included in the Report?
MtoZ Biolabs is dedicated to providing a reliable and professional Protein Acetylation Identification Service for researchers and biopharmaceutical enterprises. With advanced mass spectrometry platforms and extensive technical expertise, we help clients uncover protein modification mechanisms, facilitating disease research, biomarker development, and novel drug discovery.
Beyond acetylation, MtoZ Biolabs also offers a comprehensive portfolio of PTM identification services, including phosphorylation, methylation, ubiquitination, SUMOylation, and glycosylation. Together, these services enable the construction of systematic and complete protein modification maps, empowering both basic science and translational research. Contact us today to start your PTM research journey.
How to order?
