Peptide Mass Spectrometric Identification Service
Peptides, as pivotal biological molecules, are chains formed by amino acids linked through peptide bonds. Essential for both structural integrity and various biological functions—including hormone regulation, neurotransmission, and immune responses—peptides are critical in both health and disease.
MtoZ Biolabs is equipped with advanced mass spectrometry equipment and bioinformatics analysis tools. Combined with years of accumulated proteomics experience, we offer first-class peptide mass spectrometric identification services, achieving efficient separation and precise identification of peptides in biological samples.
Analysis Workflow
1. Sample Preparation
Proteins are extracted from samples and digested enzymatically into peptides.
2. Peptide Separation
High-performance liquid chromatography (HPLC) is employed to separate peptides.
3. Mass Spectrometry
Primary Analysis (MS): Determines the mass-to-charge ratios (m/z) of peptides.
Secondary Analysis (MS/MS): Further dissects selected peptides to decode their sequences.
4. Data Interpretation
Bioinformatics tools analyze MS/MS data against protein databases, identifying peptide sequences and modifications.
Figure 1. Analysis Workflow of Peptide Mass Spectrometric Identification
Service Advantages
Precision: Detects and precisely identifies peptides even at low concentrations.
Versatility: Effective across diverse sample types, from clinical to standard lab samples.
Efficiency: High-throughput capabilities ensure rapid processing and quick results.
Automation: Fully automated processes enhance both efficiency and accuracy.
Sample Submission Requirements
Types
Accepts liquid, solid, or gel forms.
Quantity
Minimum of 50 micrograms of protein required.
Storage
Samples must be kept frozen and protected from repeated freeze-thaw cycles prior to analysis.
Applications
· Biomarker Discovery
· Drug Design and Optimization
· Proteomics Studies
· Clinical Research
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Peptides
4. Mass Spectrometry Images
5. Raw Data
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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