O-Glycosylation Site Analysis Service | Biological Products

Unlike N-glycans, O-glycans are smaller, highly diversified branched carbohydrate molecules that can attach to various protein structures. This characteristic leads to the complexity of the analysis, making it difficult to analyze O-glycosylation sites. The variability of glycosylation sites is a key indicator of cellular activity, as evidenced by the correlation between the reduction in serum protein glycosylation sites and the severity of congenital disorders of glycosylation (CDGs). Therefore, accurately determining site occupancy is crucial for understanding the impact of protein glycosylation on human health.

 

The general steps in O-glycosylation site analysis include the proteolytic digestion of glycoproteins, enrichment and separation of glycopeptides via hydrophilic interaction liquid chromatography (HILC). Enriched O-glycopeptides can be analyzed using reversed-phase liquid chromatography-ion trap mass spectrometry (RPLC-ITMS).

 



Figure 1. General Analysis Procedure for O-glycosylation Site Attachment

 

MtoZ Biolabs has much analytical service experience in glycomics and can customize glycomics analysis services according to your specific needs. Free project evaluation!

 

Deliverables

1. Experimental Procedures

2. Mass Spectrometry Parameters

3. Mass Spectrometry Images

4. Raw Data

5. Detailed Information on Identified Glycosylation Sites