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    Biopharmaceutical Glycosylation Site Analysis Service

      Glycosylation significantly influences the three-dimensional structure, biological activity, transport, and localization of proteins. In biopharmaceutical manufacturing, particularly in the production of protein-based drugs, glycosylation is crucial for maintaining the intended structural integrity and functional performance of pharmaceuticals. Even slight variations in glycan chains can substantially affect a drug's effectiveness and safety. As biopharmaceutical innovation progresses, thorough and precise glycosylation analysis becomes critical for both manufacturers and regulatory bodies. Mass spectrometry has emerged as an effective analytical tool for glycosylation, encompassing stages from drug development to the final batch release.


      MtoZ Biolabs employs state-of-the-art mass spectrometry alongside expert bioinformatics to offer glycosylation site analysis for biopharmaceuticals. These analyses not only verify glycosylation sites within products but also assess the glycan chains' diversity and complexity, providing accurate scientific support for drug design and quality control. Additionally, our services elucidate glycosylation's specific impact on drug functions, enhancing drug efficacy and safety while meeting stringent regulatory standards.


      Analysis Workflow

      1. Sample Preparation

      Proteins are degraded to release glycopeptides.


      2. Enrichment and Separation

      Specialized enrichment columns are utilized for the effective separation of glycopeptides.


      3. Mass Spectrometry Analysis

      High-resolution mass spectrometry is employed for comprehensive analysis.


      4. Data Processing

      Advanced bioinformatics tools are used for data interpretation, ensuring results are both accurate and reliable.



      Dang, L. Y. et al. Trends Anal. Chem. 2019.

      Figure 1. Analysis Workflow of N-linked Glycoprotein Site Identification


      Services at MtoZ Biolabs

      N-glycosylation Analysis

      O-glycosylation Analysis

      Glycan Structure and Composition Analysis

      Monosaccharide Composition Analysis

      Service Advantages

      High Sensitivity: Detects even low-abundance glycosylation sites.

      High Accuracy: Ensures precise localization of glycosylation sites and a thorough analysis of glycan structures.

      Customized Services: Tailored analysis plans to meet specific customer requirements.



      Comply with ICH Q6B guidelines to ensure biopharmaceutical samples meet new market standards.

      Analyze glycosylation profiles across a variety of proteins, antibodies, and cells.

      Identify and study glycan biomarkers.

      Compare glycosylation patterns across different samples.

      Detect and characterize unusual glycosylation events.

      Maintain batch-to-batch consistency during production.



      1. Experimental Procedures

      2. Relevant Mass Spectrometry Parameters

      3. Detailed Information on Glycosylation Site

      4. Mass Spectrometry Images

      5. Raw Data


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