MW Determination Service

MW determination service involves using techniques such as mass spectrometry to accurately measure the molecular weight of molecules or compounds. This service is widely used in fields like proteomics, drug development, and chemical analysis. For proteins and other large molecules, precise molecular weight determination helps researchers confirm molecular structures, assess purity, identify different isoforms or modifications, and provide a foundation for subsequent functional analysis. In proteomics, molecular weight determination is particularly valuable for identifying target proteins, analyzing post-translational modifications (such as phosphorylation or glycosylation), and ensuring the reliability and reproducibility of experimental results. Additionally, it plays a key role in quality control, molecular validation in drug development, and purity analysis. By obtaining accurate molecular weight measurements, researchers can gain a deeper understanding of molecular characteristics, optimize product design and experimental protocols, and drive innovation and application in scientific research.

 

Service at MtoZ Biolabs

MtoZ Biolabs offers molecular weight determination services that utilize advanced chromatography and mass spectrometry technologies to provide clients with high-precision MW Determination Service. Our company is equipped with a range of cutting-edge instruments, including analytical ultracentrifuges (AUC), gel permeation chromatography (GPC), dynamic light scattering (DLS), and LC-MS systems, allowing us to accurately determine the molecular weight of various substances, including proteins, peptides, nucleic acids, small chemical molecules, and other compounds. These state-of-the-art devices ensure that our molecular weight determination achieve industry-leading levels of accuracy, sensitivity, and resolution. Whether for academic research or drug development, MtoZ Biolabs is committed to providing comprehensive and professional molecular weight determination services to support scientific innovation and product optimization. If you are interested in our services, please feel free to contact us.

 

Service Advantages

1. Exceptional Accuracy: MtoZ Biolabs ensures highly precise molecular weight determination results through advanced instruments and optimized analytical processes, meeting the demands of complex applications.

 

2. Comprehensive Technical Support: By combining multiple analytical approaches, we are equipped to address challenges across various research fields, providing flexible and effective solutions that ensure data integrity and reliability.

 

3. Extensive Experience: With years of experimental experience, MtoZ Biolabs efficiently handles a wide range of samples, maximizing the stability and reproducibility of measurements, and ensuring the reliability of results.

 

4. Customized Service Solutions: We offer tailored MW determination services based on specific client needs, whether for research projects or development applications, MtoZ Biolabs provides bespoke molecular weight determination services to help clients achieve their research objectives.

 

Sample Submission Suggestions

1. Sample Type: Clients are required to provide pure samples for analysis, which can be in liquid or solid form. Please ensure proper packaging and storage conditions based on the sample type (liquid, solution, or solid).

 

2. Sample Concentration: Please provide an adequate amount of sample to ensure multiple determinations and verify the reliability of the results. For accurate measurements, the sample concentration should meet the requirements set by our technical team. Typically, liquid samples should have a concentration between 1 μM and 10 mM. If the sample concentration is too low, please contact us to discuss whether concentration or other special treatments are needed.

 

3. Sample Purity: To ensure the accuracy and reliability of the results, it is recommended that the sample be as pure as possible. If the sample contains impurities or is in a complex matrix, it is advisable to provide a sample purity report so that we can better analyze and process the sample.

 

4. Sample Storage and Shipping: Please choose the appropriate storage and shipping method based on the characteristics of the sample. For samples that are prone to degradation or volatility, we recommend using ice packs or refrigerated shipping.

 

5. Relevant Information: Please provide basic information about the sample, including molecular structure, potential modifications, solvent information, etc., to help us tailor the best analysis plan for you.

 

Case Study

1. Investigating the molecular weight distribution of atmospheric water-soluble brown carbon using high-performance size exclusion chromatography coupled with diode array and fluorescence detectors

 



Fan, X. et al. Chemosphere. 2023.

 

2. Protein and Glycan Molecular Weight Determination of Highly  Glycosylated HIV-1 Envelope Trimers by HPSEC-MALS

 



Bender, MF. et al. Vaccine. 2021.

 

Applications

1. Analysis of Disease Biomarkers

 



Song, Y. et al. J Clin Lab Anal. 2022.

 

FAQ

1. If the sample concentration is below the required level for analysis, does it need to be concentrated? How can we determine if the concentrated sample will affect the accuracy of the molecular weight measurement?

If the sample concentration is below the required level for analysis, it is generally necessary to concentrate the sample to ensure the sensitivity and reliability of the measurement. However, it is important to note that during the concentration process, some samples may be affected by the conditions, leading to structural changes or degradation, which could impact the accuracy of the molecular weight measurement. Therefore, selecting an appropriate concentration method (such as ultracentrifugation, centrifugal filtration, dialysis, etc.) based on the specific experimental requirements is crucial, and care should be taken to avoid introducing impurities or altering the chemical properties of the sample. To ensure that the concentrated sample does not affect the accuracy of the molecular weight determination, it is recommended to perform comparative experiments before and after concentration, verifying the impact of the concentration process on the sample and ensuring the reliability of the final molecular weight results.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Molecular Weight Determination 

5. Mass Spectrometry Image

6. Raw Data