Monosaccharide Analysis Service

Monosaccharides are the simplest carbohydrates and the essential structural units of oligosaccharides, polysaccharides, and glycoconjugates. They play critical roles in metabolism, energy production, and biological recognition processes. Common sugars such as glucose, mannose, galactose, and fucose participate in key pathways of glycosylation and cellular signaling. Accurate identification and quantification of monosaccharides are fundamental for understanding glycan composition, detecting structural alterations, and monitoring product consistency in biological, food, and pharmaceutical systems.



 

Wang, Z. et al. Int. J. Biol. Macromol. 2024.

Figure 1. Main types of Monosaccharides

 

MtoZ Biolabs provides comprehensive Monosaccharide Analysis Service that combines chromatographic, spectrometric, and enzymatic platforms to deliver quantitative and structural information on a wide range of monosaccharides and their derivatives. Our analytical workflows are standardized and reproducible, ensuring data suitable for research and regulatory applications.

 

Services at MtoZ Biolabs

MtoZ Biolabs offers integrated analytical solutions for common and rare monosaccharides, sugar alcohols, amino sugars, and uronic acids.
Each project is customized based on matrix type, concentration range, and analytical purpose.

 

Glucose	Fructose	Galactose	Mannose
Xylose	Mannitol	Sorbitol	Fucose
Arabinose	Ribose	Rhamnose	Galacturonic Acid
Glucuronic Acid	Galactosamine	Mannuronic Acid	Guluronic Acid
Mannosamine	Glucosamine	Muramic Acid	N-Acetylglucosamine
Tagatose	Talose	Psicose	Sorbose

 

In addition to compositional and quantitative analysis, MtoZ Biolabs provides a specialized Monosaccharide Metabolism Analysis Service, focusing on pathway profiling, metabolic flux evaluation, and enzymatic conversion studies in biological and engineered systems.

 

Our Analytical Capabilities

MtoZ Biolabs integrates multiple analytical techniques to ensure precision, sensitivity, and reproducibility in monosaccharide characterization.

1. High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD)

HPAEC-PAD enables direct quantification of unmodified monosaccharides. It separates carbohydrates based on hydroxyl group interactions and charge distribution, providing high-resolution data without the need for derivatization. The technique is particularly effective for glycoprotein hydrolysates, fermentation media, and complex biological matrices.

 

2. High-Performance and Ultra-Performance Liquid Chromatography (HPLC/UPLC)

HPLC and UPLC systems with fluorescence or light-scattering detection allow sensitive and rapid analysis of derivatized sugars. Labeling reagents such as 2-aminobenzoic acid (2-AA) or 2-aminobenzamide (2-AB) improve detection accuracy and enable simultaneous quantification of multiple components.

3. Mass Spectrometry (MS)

LC-MS and LC-MS/MS provide detailed structural and quantitative information on monosaccharides and modified sugars. The use of isotopically labeled standards supports absolute quantification and correction for matrix effects. This approach also enables detection of phosphorylated and sulfated monosaccharides.

 

4. Capillary Electrophoresis (CE)

CE offers high-resolution separation for both charged and neutral sugars with minimal sample consumption. It is suitable for distinguishing isomers and monitoring enzymatic reactions during carbohydrate metabolism studies.

 

5. Enzymatic and Spectrophotometric Assays

Enzyme-coupled colorimetric assays provide cost-effective quantification and process monitoring for specific sugars, ensuring consistency with chromatographic and MS-based measurements.

 



 

Liu, D. et al. Food Hydrocolloids. 2021.

Figure 2. Workflow of Polysaccharide Hydrolysis and Monosaccharide Analysis Using HPAEC-PAD, HPLC, and CE

 

Sample Submission Suggestions

MtoZ Biolabs accepts biological, nutritional, and industrial samples for monosaccharide analysis.
To ensure optimal analytical performance, please follow these guidelines:

• Sample Type: Biological fluids, fermentation broths, food matrices, purified sugars, or polysaccharide hydrolysates.
• Sample Quantity: 1–5 mL of liquid or 10–20 mg of solid material.
• Storage and Shipping: Store at −20 °C and ship on dry ice to maintain sample stability.

 

Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.

 

Applications

The Monosaccharide Analysis Service supports a broad range of scientific and industrial research applications:

✅ Glycomics and Structural Research: Determination of monosaccharide composition for glycan and glycoconjugate characterization.

✅ Food and Nutrition Science: Quantification of sugars in functional foods, sweeteners, and nutritional supplements for quality verification.

✅ Pharmaceutical and Biotechnological Development: Evaluation of sugar-based excipients, therapeutic glycoproteins, and polysaccharide materials for consistency and compliance.

✅ Fermentation and Bioprocess Monitoring: Measurement of sugar consumption and conversion efficiency in microbial or enzymatic systems.

✅ Clinical and Biomedical Studies: Profiling of monosaccharide metabolism and related biomarkers in metabolic or inherited disorders

 

Why Choose MtoZ Biolabs?

1. Advanced Analytical Infrastructure

Our laboratory is equipped with LC-MS/MS, HPAEC-PAD, GC-MS, CE, and UPLC platforms suitable for comprehensive sugar analysis.

 

2. Quality and Reproducibility

All procedures follow validated protocols with internal calibration and replicate verification to ensure consistent and traceable results.

 

3. Customized Analytical Solutions

Each workflow is tailored to match the client’s research objective, whether compositional analysis, purity verification, or metabolic assessment.

 

4. Experienced Scientific Team

Our scientists possess expertise in carbohydrate chemistry and analytical biochemistry, ensuring accurate data interpretation and project support.

 

5. Transparent Data Reporting

All reports are structured, well-documented, and suitable for scientific review or industrial use.

 

FAQ

Q1: What specific monosaccharides can be analyzed with your HPAEC-PAD method?

Our HPAEC-PAD platform can accurately quantify a wide range of neutral and amino sugars, including glucose, galactose, mannose, fucose, N-acetylglucosamine, and N-acetylgalactosamine. The method offers high sensitivity and resolution without derivatization, making it suitable for both purified samples and complex biological matrices.

 

Through its scientifically rigorous and methodically validated Monosaccharide Analysis Service, MtoZ Biolabs supports researchers and industries in achieving accurate carbohydrate characterization, metabolic insight, and reliable quality assessment.

 

For more details or to discuss your project needs, contact MtoZ Biolabs today for a free consultation.

 

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