Mitochondrial Lipidomic Analysis Service

As the central organelle of intracellular energy metabolism, mitochondria play a critical role not only in oxidative phosphorylation but also in fatty acid β-oxidation, membrane structural stability, and signal transduction. The lipid composition and dynamic changes of mitochondria directly affect their functional state. Mitochondrial lipidomic analysis refers to the systematic identification and quantification of lipid species, abundance, and their variation patterns within mitochondria using high-resolution mass spectrometry and related technologies.

 

Mitochondrial lipidomic analysis service is widely applied in the study of metabolic disorders (such as diabetes and fatty liver), neurodegenerative diseases (such as Parkinson’s disease and Alzheimer’s disease), cancer, and aging. By deeply analyzing the alterations in mitochondrial lipid profiles, researchers can uncover key lipid metabolic pathways and regulatory mechanisms involved in pathological processes, providing strong support for disease prediction, target discovery, and mechanistic investigations.

 

Services at MtoZ Biolabs

Relying on high-resolution mass spectrometry platforms, MtoZ Biolabs offers a specialized mitochondrial lipidomic analysis service focused on the systematic study of mitochondrial lipids. Through optimized workflows for mitochondrial isolation and lipid extraction, we achieve high-sensitivity identification and quantification of various lipid species, including phospholipids, sphingolipids, and glycerolipids. The service delivers multidimensional data covering lipid classes, abundance, and structural configurations, supporting lipid metabolism pathway analysis and differential lipid screening, and helping to elucidate the critical roles of mitochondrial lipids in energy regulation, cellular function, and disease mechanisms.

 

Analysis Workflow

1. Mitochondrial Isolation

Differential centrifugation and density gradient methods are employed to isolate highly purified mitochondria from cells or tissues, ensuring specificity and accuracy in lipidomic analysis.

 

2. Lipid Extraction

Extraction protocols suitable for both polar and non-polar lipids are used under low-temperature conditions to stably recover mitochondrial lipids while minimizing degradation and oxidation.

 

3. Mass Spectrometry Detection

Lipid species and abundances are profiled using LC-MS/MS or GC-MS platforms, supporting both targeted and untargeted analysis strategies.

 

4. Data Analysis

Lipid profiles are constructed through database matching and multivariate statistical analysis, followed by differential analysis and pathway enrichment to reveal biological relevance and functional alterations.

 

Sample Submission Suggestions

1. Sample Types

We accept samples derived from animal tissues, cell lines, and plant materials. Purified mitochondrial fractions are preferred. If needed, we can assist with mitochondrial isolation from whole cells or tissues.

 

2. Sample Storage and Transport

Samples should be snap-frozen in liquid nitrogen immediately after processing and stored at −80°C. Please use dry ice during transportation to avoid freeze-thaw cycles and minimize lipid oxidation or degradation.

 

3. Additional Notes

If samples have undergone prior processing, please specify the buffer systems and procedures used. For any uncertainties regarding sample types or preparation protocols, we encourage early consultation with our technical team.

 

Service Advantages

1. High-Purity Mitochondrial Extraction

Utilizing differential centrifugation and density gradient separation, we ensure intact mitochondrial structure with minimal contamination—laying a solid foundation for accurate lipid analysis.

 

2. Advanced Analytical Platforms

Built on high-resolution mass spectrometry systems, our platform accommodates both polar and non-polar lipids, enabling comprehensive qualitative and quantitative profiling.

 

3. Comprehensive Lipidome Coverage

Capable of analyzing key lipid classes including phospholipids, sphingolipids, and glycerolipids, supporting studies on mitochondrial membrane integrity, functionality, and lipid metabolism dynamics.

 

4. Standardized End-to-End Workflow

From sample preparation and lipid extraction to data interpretation, we offer a streamlined service pipeline that guarantees reproducibility, scientific rigor, and operational efficiency for our clients.

 

Applications

1. Mitochondrial Dysfunction Mechanism Research

By analyzing changes in the mitochondrial lipidome, this approach reveals the role of lipids in maintaining membrane integrity, regulating signaling pathways, and facilitating energy conversion, contributing to the understanding of dysfunction mechanisms.

 

2. Metabolic Disease Research

Mitochondrial lipidomic analysis service is applicable to the investigation of mitochondrial lipid metabolism abnormalities in diseases such as diabetes, obesity, and non-alcoholic fatty liver disease, aiding in the discovery of potential biomarkers or therapeutic targets.

 

3. Neurodegenerative Disease Exploration

By studying alterations in mitochondrial membrane lipid composition in diseases like Alzheimer’s and Parkinson’s, this service uncovers the regulatory role of lipids in neurodegenerative processes.

 

4. Oxidative Stress and Aging Mechanism Research

Mitochondrial lipidomic analysis service can be used to uncover the critical role of lipid oxidation in cellular aging, apoptosis, and stress responses, supporting research into anti-aging and homeostatic regulation.

 

FAQ

Q1: Is it Possible to Perform Mitochondrial Lipidomic Analysis Using Frozen Samples?

A1: Yes. To ensure the integrity and stability of lipids, we recommend flash-freezing samples in liquid nitrogen and storing them at −80°C. During transportation, samples should be shipped on dry ice and protected from repeated freeze-thaw cycles.

 

Q2: Can Proteomics or Metabolomics Be Analyzed in Parallel with Lipidomics?

A2: Yes. We can perform integrated multi-omics analysis of mitochondria, including lipidomics, metabolomics, and proteomics, according to the client’s research needs, facilitating deeper investigation into underlying biological mechanisms.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Data Analysis, Preprocessing, and Estimation

4. Bioinformatics Analysis

5. Raw Data Files