Metabolomics FAQ

• • What Does the VIP Score Represent in the OPLS-DA Model

  Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) is a multivariate statistical method widely used for pattern recognition and classification. In the OPLS-DA model, the Variable Importance in the Projection (VIP) score serves as a key metric for assessing the relative importance of each variable in the model.   Interpretation of the VIP Score 1. The VIP score quantifies the contribution of a variable to the model’s predictive performance and classification accuracy.   2. A higher VIP sc......

• • How to Collect Mouse Serum Samples

  The procedures for collecting mouse serum samples: 1. Blood Collection: Blood samples are collected from the mouse's tail vein or jugular vein. 2. Coagulation: The collected blood is allowed to clot at room temperature for approximately 15-30 minutes. 3. Centrifugation: The clotted blood is centrifuged at 3000-5000 g for 10-15 minutes to separate the serum from the clot. 4. Serum Collection: The serum is transferred to a clean centrifuge tube and stored at -20°C or -80°C for subsequent analyses.