How to Collect and Process Blood Samples for Metabolomics Testing
Blood Sample Collection
1. Collection Time
It is best to collect blood samples at the same time point (e.g., in the morning on an empty stomach) to minimize the impact of biological rhythms.
2. Collection Method
Venipuncture is typically used with sterile collection tubes, which may contain or lack anticoagulants depending on the subsequent processing and analysis requirements.
3. Sample Volume
Usually, 1-2 mL of blood is required.
Blood Sample Processing
1. Centrifugation
Centrifuge the blood samples at 4°C (e.g., 3000 g for 10 minutes) to separate plasma or serum.
(1) Plasma: Centrifuge immediately after blood collection at 4°C (e.g., 3000 g for 10 minutes) to separate plasma. Plasma is located at the top of the tube and should be transferred carefully to avoid disturbing red blood cells.
(1) Serum: Allow blood to clot naturally at room temperature (about 30 minutes), then centrifuge at 4°C (e.g., 3000 g for 10 minutes). Serum is located at the top of the tube and should be separated carefully.
2. Sample Aliquoting
Transfer the separated serum or plasma into new sterile tubes and label them.
The separated plasma or serum should be stored at low temperatures, typically at -80°C, to prevent metabolite degradation. If the samples need to be sent to another location for analysis, they should be transported under cold chain conditions.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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