Metabolites Molecular Weight Measurement Service
- High Precision: ppm-level mass accuracy ensures confident molecular weight determination for a broad range of metabolites.
- Rapid Turnaround: streamlined sample preparation and optimized workflows enable timely delivery of results without compromising quality.
- Customizable Solutions: flexible analytical strategies allow us to tailor methods for different metabolite classes, research goals, and sample types.
- Comprehensive Coverage: suitable for diverse metabolites, including primary intermediates, secondary metabolites, and complex mixtures.
- Reproducibility: standardized protocols and strict quality control guarantee consistent, publication-ready data.
- Comprehensive Experimental Details
- Materials, Instruments, and Methods
- Mass Spectrometry Images
- Data Analysis, Preprocessing, and Estimation (project-dependent)
- Bioinformatics Analysis
- Raw Data Files
MtoZ Biolabs provides a professional Metabolites Molecular Weight Measurement Service to deliver accurate, reliable, and reproducible data for metabolites across biological, pharmaceutical, agricultural, and industrial applications. By integrating advanced mass spectrometry platforms with optimized workflows, our service enables precise molecular weight determination of diverse metabolites, including small molecules, intermediates, and metabolic products.
Metabolites are central players in the biochemical network of life. They include amino acids, organic acids, nucleotides, sugars, fatty acids, and secondary metabolites such as alkaloids and flavonoids. Measuring their molecular weight with accuracy is the foundation for structural elucidation, compound identification, biomarker discovery, and metabolic pathway analysis. The molecular weight of a metabolite provides key information about its chemical identity, possible structural variants, and its role in physiological and pathological processes.
As metabolic research expands into precision medicine, nutrition science, environmental studies, and industrial biotechnology, accurate molecular weight measurement has become indispensable. Among available analytical approaches, mass spectrometry has emerged as the most suitable and widely adopted method for this task. Its high sensitivity, excellent mass accuracy, and ability to handle complex biological mixtures make it ideal for capturing the molecular weight of metabolites across diverse chemical classes.
Service at MtoZ Biolabs
MtoZ Biolabs' dedicated Metabolites Molecular Weight Measurement Service integrates advanced mass spectrometry platforms with optimized sample workflows to ensure the highest accuracy and reproducibility.
The process begins with careful sample preparation to remove salts, detergents, and other interfering substances that may suppress ionization. Once prepared, metabolites are introduced into the mass spectrometer using ionization techniques such as electrospray ionization or matrix-assisted laser desorption ionization, which are well suited for small molecules and complex mixtures. Following ionization, analytes are transferred into high-resolution mass analyzers where they are separated according to their mass-to-charge ratios. These measurements, calibrated with internal and external standards, are then deconvoluted to obtain precise neutral molecular weights.
By combining accurate m/z detection with isotopic distribution analysis, our approach provides both molecular weight determination and supporting evidence for compound identity. For metabolites with potential structural variants or isomeric forms, tandem mass spectrometry can be employed to generate fragmentation spectra that strengthen confidence in assignment. In addition, liquid chromatography or gas chromatography can be integrated upstream of mass spectrometry when samples are particularly complex, ensuring that metabolites are measured free of co-eluting interferences.
Huang, W. T. et al. Molecules. 2012.
Figure 1. MALDI-TOF MS Analysis of the Molecular Weights of Polysaccharides and Their Derivatives
Analysis Workflow
Service Advantages
Sample Submission Suggestions
1. Sample Types
Tissues, biofluids such as serum, plasma, urine, cerebrospinal fluid, cultured cells or lysates, microbial cultures, plant tissues, and purified extracts or fractions.
2. Recommended Amounts
Typically, at least 200 μL of liquid sample or 200 mg of solid sample is required. Final quantities will be confirmed during project consultation.
3. Sample Condition
Samples should be free of detergents, salts, and other contaminants that may interfere with ionization.
4. Storage and Transport
Biological samples should be frozen at –80°C and shipped on dry ice. Minimize freeze–thaw cycles.
5. Documentation
Please provide detailed information on sample composition, concentration, and any prior analytical data to facilitate optimal analysis.
Clients are encouraged to contact MtoZ Biolabs for customized sample preparation advice.
Deliverables
MtoZ Biolabs' Metabolites Molecular Weight Measurement Service is designed to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.
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