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    MALDI-TOF Microbial Identification Service

      MALDI-TOF microbial identification is a highly efficient analytical technique based on mass spectrometry that allows for the rapid and accurate identification of bacteria, fungi, and other microorganisms through their protein fingerprints. Utilizing Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), this method ionizes microbial proteins or lipids, producing a mass spectrum unique to the species based on ion mass and abundance, which is then used for species identification.

       

      Compared to traditional biochemical kits and gene amplification methods, MALDI-TOF microbial identification significantly reduces identification time, simplifies operations, and enhances accuracy. Whether for clinical diagnostics, environmental monitoring, or food safety testing, the MALDI-TOF Microbial Identification Service delivers quick, reliable microbial analysis. This technology typically provides identification results within minutes, greatly enhancing experimental efficiency and meeting the high demands for both speed and accuracy in various application scenarios.

       

      MtoZ Biolabs offers highly efficient and precise MALDI-TOF Microbial Identification Service leveraging state-of-the-art mass spectrometry platform. Our services deliver reliable results across diverse fields, including scientific research, clinical diagnostics, food safety testing, and public health research.

       

      Analysis Workflow

      The MALDI-TOF Microbial Identification Service at MtoZ Biolabs is optimized to ensure high efficiency and accuracy at every step:

      1. Sample Collection and Preparation

      Samples can be obtained either via microbial cultivation or directly from environmental or clinical specimens. To ensure accuracy, we provide detailed guidelines for sample preparation.

       

      2. Mass Spectrometry Analysis

      The samples are analyzed using MALDI-TOF MS, where proteins are ionized via matrix-assisted laser desorption and analyzed based on their mass-to-charge ratio.

       

      3. Data Comparison and Species Identification

      The generated mass spectra are compared against a vast database of microbial fingerprints, ensuring fast and accurate species identification.

       

      4. Result Report

      Upon completion, a detailed report with identification results, species information, and database comparison outcomes is provided. We also offer clear interpretations of the report for effective application of the findings.

       

      1882711954117218304-maldi-tof-microbial-identification-service1.png 

      Jang, K. et al. J. Microbiol. 2018.

      Figure 1. Workflow for Microbial Identification using MALDI-TOF MS

       

      Service Advantages

      1. Rapid and Efficient

      Our MALDI-TOF Microbial Identification Service delivers results in just minutes, significantly enhancing diagnostic and testing efficiency. It is particularly suited for time-sensitive applications in clinical emergencies, food safety, and environmental monitoring.

       

      2. High Accuracy and Reliability

      Leveraging protein fingerprinting and a comprehensive microbial database, our service provides highly accurate microbial identification, even in complex or heterogeneous samples, ensuring consistent reliability.

       

      3. Broad Applicability

      MALDI-TOF Microbial Identification Service is suitable for identifying bacteria, fungi, and other microorganisms and is widely applicable across various sectors, including clinical microbiology, food safety, environmental monitoring, and more, providing comprehensive solutions for different industries.

       

      4. Tailored Solutions

      We offer customized MALDI-TOF Microbial Identification Service to meet the specific needs of each project, ensuring precise execution while providing comprehensive technical support and consultation throughout the process.

       

      5. One-Time-Charge

      Our pricing is transparent, no hidden fees or additional costs.

       

      Sample Submission Suggestions

      To ensure the accuracy and reliability of the analysis results, please make sure your samples meet the following criteria:

      • Sample Type: Suitable for a wide range of microorganisms, including bacteria, fungi, environmental samples, and clinical specimens.
      • Sample Collection: Samples can be obtained through microbial cultivation or directly from clinical or environmental sources.
      • Sample Quantity: Both individual and bulk samples can be processed, depending on the experimental needs.
      • Sample Storage: Ensure that samples are stored at low temperatures and are protected from exposure to high temperatures or humid environments.

      MtoZ Biolabs provides detailed guidelines for sample submission to ensure that the samples meet the required standards, thus enhancing the reliability of the analysis results. Please feel free to contact us for further assistance.

       

      Applications

      The MALDI-TOF Microbial Identification Service has broad applications across various fields, including:

      • Clinical Microbiology: Rapid identification of pathogenic microorganisms to assist in clinical diagnosis and treatment.
      • Environmental Monitoring: Microbial identification in environmental samples such as water, air, soil, and others.
      • Food Safety: Microbial testing in food products to ensure safety and quality control.
      • Pharmaceutical Industry: Detection of microbial contamination in production environments to maintain sterile conditions in manufacturing processes.

       

      Case Study

      Case 1: Application of MALDI-TOF MS in Microbial Identification and Antibiotic Resistance Detection

      This study utilized MALDI-TOF MS to rapidly identify microorganisms and detect antibiotic resistance by analyzing bacterial membrane lipids. The results demonstrated that MALDI-TOF MS is highly effective in distinguishing different microbial species and identifying lipid markers related to antibiotic resistance. This method not only accelerates the speed and accuracy of microbial identification but also supports resistance monitoring, highlighting the potential of MALDI-TOF Microbial Identification Service in clinical microbiology and public health research.

       

      1882712197583982592-maldi-tof-microbial-identification-service2.png

      Liang, T. et al. Anal. Chem. 2018.

       

      Case 2: Integration of MALDI-TOF MS and Machine Learning to Improve Microbial Identification

      This research combined MALDI-TOF MS with machine learning algorithms to differentiate Weissella cibaria from Weissella confusa. By analyzing the mass spectrometry data and applying machine learning classification techniques, the study enhanced the accuracy and efficiency of microbial species differentiation. This case demonstrates the powerful capabilities of MALDI-TOF Microbial Identification Service in improving the accuracy of microbial identification and provides a rapid, precise solution for microbial classification.

       

      1882712298813509632-maldi-tof-microbial-identification-service3.jpg 

      Kim, E. et al. Int. J. Mol. Sci. 2023.

       

      Deliverables

      1. Comprehensive Experimental Details

      2. Materials, Instruments, and Methods

      3. Total Ion Chromatogram & Quality Control Assessment

      4. Data Analysis, Preprocessing, and Estimation

      5. Bioinformatics Analysis

      6. Raw Data Files

       

      Choose MtoZ Biolabs' MALDI-TOF Microbial Identification Service for more precise and efficient microbial identification. Contact us to take advantage of our expert technical support and customized services tailored to your specific needs.

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