Lysine β-hydroxybutyrylation Proteomics Analysis Service

Lysine β-hydroxybutyrylation ((Kbhb) proteomics analysis offers a systematic approach to characterizing this emerging post-translational modification through high-resolution mass spectrometry and targeted enrichment strategies. This platform enables comprehensive profiling of Kbhb site distribution, temporal dynamics, and functional associations across diverse biological states—making it a valuable tool for elucidating the crosstalk between metabolic stress, epigenetic remodeling, and intracellular signaling.

 

Kbhb, catalyzed by the ketone body metabolite β-hydroxybutyrate, has been increasingly recognized for its regulatory roles in chromatin organization, transcriptional control, DNA repair, and metabolic homeostasis. Its dynamic, enzyme-regulated, and tissue-specific features position it as a critical nexus between metabolism and epigenetic regulation.

 

MtoZ Biolabs provides high-quality Lysine β-Hydroxybutyrylation Proteomics Analysis Service powered by advanced mass spectrometry platforms, optimized antibody-based enrichment, and integrative bioinformatics. Our service supports deep exploration of Kbhb modification landscapes, underlying regulatory pathways, and candidate targets—empowering researchers to bridge basic science with translational applications.

 

Services at MtoZ Biolabs

At MtoZ Biolabs, our Lysine β-Hydroxybutyrylation Proteomics Analysis Service delivers an end-to-end solution for Kbhb research, supporting investigations across metabolic, oncological, neurological, and epigenetic landscapes.

·  High-specificity antibody enrichment

Capture low-abundance Kbhb-modified peptides using optimized antibodies to maximize detection sensitivity

 

·  Bottom-up proteomics

Identify modification sites at the peptide level using high-resolution LC-MS/MS after enzymatic digestion

 

·  Top-down proteomics

Analyze intact proteins to resolve co-existing modification patterns and interpret site-level heterogeneity

 

·  Flexible quantification strategies

Supports TMT labeling, label-free quantification, and semi-quantitative methods depending on experimental needs

 

·  Subcellular localization (optional)

Visualize Kbhb spatial distribution via immunofluorescence or immunohistochemistry

 

·  Bioinformatics interpretation

Includes GO and KEGG enrichment, sequence motif preference, clustering analysis, and protein interaction network mapping

 

 

Figure 1. Lysine β-hydroxybutyrylation Proteomics Analysis Workflow

 

Service Advantages

✔ Dual-modality MS profiling: Integrates both bottom-up and top-down strategies for complete Kbhb landscape mapping

✔ Low-input compatibility: Suitable for clinical or precious samples with microgram-level input

✔ Quantification with depth and flexibility: Offers scalable options tailored to project goals and budget

✔ Interpretation beyond identification: Functional annotations translate raw data into biologically actionable insights

✔ Expert-guided delivery: From design to report, our team ensures publishable-quality results and technical consultation

 

Sample Submission Suggestions

1. Sample Types

Serum, plasma, urine, tissue extracts, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Shipping

Dry ice shipment required; avoid repeated freeze–thaw.

 

Note: SDS-free, MS-compatible lysis buffer is preferred; contact us for protocol support

 

Applications

Our Lysine β-Hydroxybutyrylation Proteomics Analysis Service is ideal for dissecting the intersection of metabolic and epigenetic regulation in the following research domains:

1. Metabolic pathway remodeling: Investigate Kbhb responses in models of fasting, ketogenesis, or caloric restriction

2. Cancer epigenomics: Elucidate how Kbhb modulates tumor-specific chromatin states and gene expression

3. DNA damage response: Explore dynamic Kbhb changes on repair proteins and reconstruct modification networks

4. RNA processing regulation: Profile Kbhb involvement in splicing machinery and nuclear transport factors

5. Neurobiology of disease: Identify brain-region-specific Kbhb patterns in epilepsy, neuroinflammation, or cognitive decline

6. Preclinical target discovery: Screen for Kbhb-regulated pathways or protein candidates for drug development

 

Case Study

Case 1：Uncovering the Role of β-Hydroxybutyrylation in Hepatic Metabolic Regulation via Mass Spectrometry

A landmark study (Cell Reports, 2021) employed mass spectrometry to characterize the hepatic Kbhb landscape under ketogenic conditions. Researchers identified 891 Kbhb sites across 267 proteins, mainly enriched in fatty acid oxidation, amino acid metabolism, and detoxification pathways.

 

One key discovery was that Kbhb modification inhibited the enzymatic activity of AHCY—a critical enzyme in the methionine cycle—demonstrating how Kbhb directly alters enzyme function and metabolic flux. This underscores Kbhb’s dual role in both epigenetic regulation and metabolic control.

 

 

Koronowski, K. B. et al. Cell Rep. 2021.

 

MtoZ Biolabs' Lysine β-Hydroxybutyrylation Proteomics platform enables comparable profiling and functional discovery across tissues and disease models.

 

FAQ

Q1: Is this service suitable for small or rare sample quantities?

Yes. We support inputs as low as microgram-level protein and offer automated nano-scale enrichment compatible with low-yield clinical or neurological samples.

 

Q2: How do I choose between bottom-up and top-down strategies?

Bottom-up is ideal for high-throughput profiling and quantitation; top-down excels in revealing PTM combinations and isoform complexity. Our specialists will guide you based on your biological question and sample type.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

At MtoZ Biolabs, we are committed to advancing post-translational modification research through precision proteomics. The Lysine β-hydroxybutyrylation Proteomics Analysis Service offers a powerful framework to explore, quantify, and decode the regulatory landscape of Kbhb. Contact us today to discuss your project and receive a tailored analysis plan.