Is Yellow Coloration of Intestinal Tissue Lysate Supernatant Normal During Western Blot Sample Preparation?

In the context of Western blotting, it is common for the supernatant obtained from intestinal tissue protein extraction to exhibit a yellow coloration. This is typically due to the presence of bile pigments naturally found in intestinal tissue, which can impart a yellow hue to the lysate.

 

Importantly, the color of the lysate does not directly influence Western Blot outcomes. Instead, the reliability of the experiment primarily depends on the efficiency of protein extraction and the accuracy of concentration determination. However, if other unusual observations are noted or if the experimental results are suboptimal, it is advisable to reassess the extraction protocol and the quality of reagents used.

 

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

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