Is There a Association Between SDS-PAGE Band Smearing and Protein Glycosylation?
- Degradation of the protein sample
- Suboptimal electrophoresis conditions (e.g., buffer composition, applied voltage, and electrophoresis duration)
- Gel-related issues, such as incomplete polymerization or inappropriate gel temperature
- Excessive heating of the sample or insufficient denaturation before electrophoresis
- Overloading of the sample
Yes, the diffusion or smearing of SDS–PAGE bands can be potentially associated with protein glycosylation. Glycosylation can introduce heterogeneity into protein samples, which refers to the occurrence of identical proteins in multiple glycoform variants. Such structural variability may result in the appearance of multiple discrete bands or diffuse bands during SDS–PAGE analysis. Furthermore, glycosylation can alter the interaction between proteins and sodium dodecyl sulfate (SDS), thereby affecting their migration behavior within the gel matrix.
It should be noted, however, that SDS–PAGE band diffusion can also arise from other factors, including but not limited to:
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Submit Inquiry
How to order?
