Is Dialysis Necessary During Polysaccharide Extraction?
Yes, dialysis—often conducted under running water—is typically employed during the polysaccharide extraction process. This step is essential for removing low-molecular-weight impurities, such as salts, small organic compounds, and low-molecular-weight proteins, that may be introduced or remain after extraction. In addition, dialysis facilitates the purification and concentration of the polysaccharide samples, thereby enhancing their overall purity and quality. The selection of an appropriate dialysis membrane should be guided by its molecular weight cut-off (MWCO) to ensure efficient separation of target polysaccharides from smaller contaminants.
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