Is Coomassie-Stained SDS-PAGE Gel Suitable for Protein Mass Spectrometry Following Western Blot Analysis?
It is theoretically feasible to perform protein mass spectrometry (MS) analysis on SDS-PAGE gels stained with Coomassie Brilliant Blue, even when used in the context of Western blot experiments. However, to ensure optimal enzymatic digestion and accurate MS results, it is essential that the stained proteins are thoroughly destained, and that no residual dye remains within the gel matrix. Incomplete destaining may interfere with peptide recovery and signal quality, ultimately compromising spectral clarity and identification accuracy.
For optimal results, extensive destaining should be performed following Coomassie staining to minimize potential interference with downstream mass spectrometric procedures.
Comprehensive protein mass spectrometry services are available through BGI-Tech or similar biotechnology platforms, which offer expertise in handling complex biological samples and delivering high-confidence MS-based protein identifications.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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