Intact Protein Mass Spectrometry Service

Intact Protein Mass Spectrometry Service uses highly sensitive mass spectrometry technology to analyze the molecular mass, composition, post-translational modifications, and higher-order structural features of intact proteins, maintaining their native and unaltered state. Intact protein mass spectrometry (IP-MS) plays a crucial role in understanding protein integrity, identifying sequence variations, and characterizing modifications that may impact protein function or stability. In biopharmaceutical development, where protein purity and quality are critical, IP-MS offers significant advantages; in basic research, it aids in understanding protein conformations and interactions. By providing precise and comprehensive analysis of intact proteins, IP-MS supports accurate biomarker discovery, therapeutic protein development, and more efficient drug design. This technology offers direct and detailed insights into protein structure and modifications, enhancing our understanding of complex biological systems and driving innovation in both research and industry.

 

Service at MtoZ Biolabs

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. We have established a powerful and professional intact protein analysis platform, which includes Thermo Fisher Q Exactive HF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our service include intact protein molecular weight determination, polymer-protein complex molecular weight determination, protein purity and integrity assessment, protein degradation and stability analysis, and peptide mapping and sequence analysis, among others. With an experienced team and advanced equipment, MtoZ Biolabs provides high-quality, reliable analytical results to support scientific discovery and innovative research. If you are interested in our services, please feel free to contact us.

 

Analysis Workflow

 



 

Service Advantages

1. High Accuracy and High Resolution: Using advanced mass spectrometry techniques (such as MALDI-TOF or ESI-MS), we provide precise protein molecular weight determination, ensuring accurate identification of proteins and their subtypes.

 

2. Fast and Efficient: With an efficient analytical workflow, high-quality molecular weight data can be obtained in a short amount of time, making it suitable for large-scale screening and rapid identification.

 

3. Applicable to a Wide Range of Samples: Whether for purified proteins or complex samples, we can provide precise molecular weight determination, meeting the needs of various research fields.

 

4. High Sensitivity: Even low-abundance protein samples can be accurately detected, catering to a wide range of research needs from basic science to clinical studies.

 

5. Supports Modification Analysis: In addition to routine molecular weight determination, we can also detect the impact of post-translational modifications (such as phosphorylation, glycosylation, etc.) on protein molecular weight, aiding in the analysis of complex protein structures.

 

Sample Submission Suggestions

1. Sample Purity: The sample should be purified as much as possible to minimize impurities that could interfere with mass spectrometry analysis. Purity is generally required to be above 80%.

 

2. Sample Concentration: It is recommended to provide protein samples at a concentration typically between 1-10 μM, with specific requirements adjustable based on sample type and analysis method.

 

3. Solvent Requirements: Samples should be dissolved in a buffer suitable for mass spectrometry analysis, such as deionized water, PBS, or other appropriate salt-free buffers. Avoid using solvents containing organic compounds or solutions with excessively high salt concentrations.

 

4. Sample Storage: If the sample cannot be analyzed immediately, it should be stored at -80°C or -20°C and kept from repeated freeze-thaw cycles to maintain protein stability.

 

5. Special Requirements: For post-translationally modified proteins or complexes, additional information such as the type of modification or complex composition may be required to ensure optimal analysis results.

 

Case Study

1. Native Ambient Mass Spectrometry of an Intact Membrane Protein Assembly and Soluble Protein Assemblies Directly from Lens Tissue

 



Hale, OJ. et al. Angew Chem Int Ed Engl. 2022.

 

2. Intact mAb LC–MS for Drug Concentration from Pre-Clinical Studies: Bioanalytical Method Performance and In-Life Samples

 



Kellie, JF. et al. Bioanalysis. 2020. 

 

Applications

1. Used For Drug Discovery

 



Pu, F. et al. Anal Chem. 2022. 

 

FAQ

1. Does the solvent used interfere with mass spectrometry analysis, and how can the compatibility of the solvent with the mass spectrometer be ensured to avoid affecting the molecular weight determination results?

The choice of solvent is critical for mass spectrometry analysis, as certain solvents or solvents may interfere with the instrument, affecting the signal intensity and quality, and even damaging the equipment. To ensure solvent compatibility, deionized water, PBS, or other low-salt, low-volatility solvents should be used, avoiding high-concentration salt solutions or organic solvents. After dissolving the sample, check for impurities or bubbles in the solvent to ensure the stability and accuracy of the mass spectrometry analysis. Using the appropriate solvent can reduce background noise and ensure the reliability of molecular weight determination results.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Intact Protein Analysis 

5. Mass Spectrometry Image

6. Raw Data