Inhibitor Binding Site Determination Service

Precise identification of inhibitor binding sites is essential in drug discovery and mechanism-of-action studies. Determining how inhibitors interact with target proteins reveals regulatory mechanisms in signaling pathways, accelerates lead optimization, and provides structural insights for innovative therapeutic strategies. Advances in mass spectrometry and structural biology now allow binding site mapping at atomic or residue-level resolution through integrated approaches.

 

MtoZ Biolabs offers reliable Inhibitor Binding Site Determination Service powered by advanced mass spectrometry platforms and multi-dimensional experimental strategies. We deliver reproducible data and functional insights that support both academic research and pharmaceutical development.



Zhang, X. et al. J Enzym Inhib Med Ch. 2021.

Figure 1. Inhibitor Binding Site Determination Workflow

 

Services at MtoZ Biolabs

MtoZ Biolabs’ Inhibitor Binding Site Determination Service delivers end-to-end solutions, leveraging high-resolution LC-MS/MS and advanced experimental strategies to provide precise and reliable mapping of inhibitor binding sites.

● Covalent Inhibitor Mapping: Detect mass shifts and use MS/MS fragmentation to pinpoint modified residues with high accuracy.

● Non-covalent Inhibitor Mapping: Apply HDX-MS, covalent labeling MS, and XL-MS to analyze conformational and accessibility changes and indirectly map binding regions.

● Multi-dimensional Validation: Integrate molecular docking, molecular dynamics, and mutagenesis experiments to confirm results with strong interpretability.

 

Why Choose MtoZ Biolabs?

✅ High-resolution Platforms: Orbitrap Fusion Lumos, Q Exactive HF, and TOF-MS deliver precise mapping.

✅ Advanced Labeling Strategies: Antibody capture, photoaffinity probes, K-ε-GG labeling, and covalent footprinting.

✅ Broad Sample Compatibility: Supports purified proteins, complexes, cells, tissues, blood, exosomes, and recombinant proteins.

✅ Expert Interpretation: Delivers annotated spectra, pathway analyses, and functional reports that drive research forward.

✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

Sample Submission Suggestions

·  Accepted samples: Purified proteins, complexes, cell lysates, and tissue extracts.

·  Storage: Keep at –80°C, avoid freeze-thaw cycles.

·  Transport: Ship on dry ice.


Note: Provide details on sample collection and handling. If you need further details, our technical support team is happy to assist and provide comprehensive guidance on sample submission.

 

Applications

·  Drug Discovery: Identify binding sites of small-molecule inhibitors to optimize leads.

·  Target Validation: Confirm binding features of potential targets for novel therapies.

·  Mechanism Studies: Uncover how inhibitors regulate protein function and pathways.

·  Structural Biology: Combine with cryo-EM or X-ray crystallography to refine complex models.

·  Biologics Development: Map binding sites to enhance specificity and stability of antibodies and biologics.

 

MtoZ Biolabs Inhibitor Binding Site Determination Service provides high-confidence data that accelerates drug development, target validation, and mechanistic research. With cutting-edge MS platforms, integrated strategies, and expert data analysis, we help researchers and pharmaceutical teams move from discovery to innovation with confidence.