HPLC Peptide Purity Analysis Service

High performance liquid chromatography (HPLC) is a modern chromatographic separation technique extensively utilized in biological, chemical, and medical research. For peptide purity analysis, HPLC identifies the composition and content of peptides in samples, assessing the purity and impurities. Peptides are dissolved in a mobile phase and separated using a chromatographic column containing a stationary phase. Different components in the peptide sample exhibit distinct retention times based on their interactions. The detector analyzes retention time and signal intensity to qualitatively and quantitatively assess peptide purity.

 

Analysis Workflow

1. Sample Preparation

Dissolve peptide samples in an appropriate solvent (e.g., water, acetonitrile, methanol) to form the test solution.


2. Set HPLC Conditions

Adjust parameters like mobile phase, flow rate, gradient program, and detection wavelength according to sample characteristics and the chromatographic column.


3. System Equilibration

Flow the mobile phase through the column until reaching a stable equilibrium to ensure reproducible results.


4. Sample Injection

Introduce the test solution to the chromatography system using an automatic or manual injector.


5. Separation Process

Peptide samples are carried through the column by the mobile phase, separating components based on their interaction with the stationary phase.


6. Detection and Data Collection

The detector sequentially registers each peptide component passing through, producing electrical signals. The system captures retention time and signal intensity to create chromatograms.


7. Data Processing and Analysis

Specialized software processes chromatograms, providing qualitative and quantitative peptide purity analysis based on retention time and signal intensity.

 



 

Applications

1. Drug Development and Preparation

Assess peptide drug purity, composition, impurities, and concentration to ensure drug quality and safety while enabling high-purity peptide preparation.


2. Industrial Production

Monitor purification steps, degradation, and impurity production during peptide manufacturing to guide process optimization and quality control.


3. Clinical Diagnosis

Measure peptide levels in biological samples (e.g., serum, urine) to support clinical diagnosis, disease monitoring, and treatment evaluation.


4. Food Safety

Confirm peptide additive and residue purity in food, ensuring safety and quality.


5. Environmental Monitoring

Detect peptide pollutants in environmental samples (e.g., water, soil) to aid environmental risk assessment and pollution control.

 

Sample Submission Requirements

 



 

Deliverables

In the technical report, MtoZ Biolabs will provide you with a detailed technical information, including:

1. Experimental Procedures

2. Relevant Chromatographic Parameters

3. HPLC Peptide Purity Analysis Detailed Information

4. Chromatography Images

5. Raw Data