How to Collect Mouse Serum Samples
The procedures for collecting mouse serum samples:
1. Blood Collection
Blood samples are collected from the mouse's tail vein or jugular vein.
2. Coagulation
The collected blood is allowed to clot at room temperature for approximately 15-30 minutes.
3. Centrifugation
The clotted blood is centrifuged at 3000-5000 g for 10-15 minutes to separate the serum from the clot.
4. Serum Collection
The serum is carefully extracted from the top layer to avoid disturbing the underlying red blood cells or clot.
5. Storage
The serum is transferred to a clean centrifuge tube and stored at -20°C or -80°C for subsequent analyses.
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