How Can The Content And Structure Of Plant Polysaccharides Be Determined Directly By Mass Spectrometry?
Content Determination
The quantification of polysaccharides was performed using liquid chromatography-mass spectrometry (LC-MS). First, polysaccharides in the sample were separated by liquid chromatography (LC). Subsequently, the separated polysaccharides entered the mass spectrometer, where the molecules were ionized to form ions. By measuring the mass-to-charge ratios (m/z) of the polysaccharide ions, the concentration of polysaccharides was quantified. This quantification generally requires comparison with known standard references to ensure accuracy.
Structural Analysis
The structural characterization of polysaccharides was conducted using LC-MS. The mass spectrometer was used to measure the molecular weights of the polysaccharides. Furthermore, the polysaccharides produced fragment ions within the mass spectrometer, and the analysis of these fragment ion spectra provided structural information. By comparing these data with established polysaccharide structure databases or interpreting the fragmentation patterns based on chemical knowledge, the structures of the polysaccharides were elucidated.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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