Glycosylation Sites and Glycoform Analysis Service

    Protein glycosylation not only influences the protein's spatial configuration, biological activity, transportation, and localization, but also plays a crucial role in specific biological processes such as molecular recognition, cell communication, and signal transduction. Therefore, the analysis of glycosylation sites and their glycoforms is of great significance.

     

    MtoZ Biolabs offers a glycosylation site and glycoform analysis service based on LC-MS/MS. We typically start by selecting various enzymes to digest the target protein to cover as much of the protein sequence as possible. After cleaving the glycans by enzymes, the glycosylation sites in the protein are analyzed. Once the glycosylated peptides are identified, the target protein is redigested and the glycans on the peptides are retained. During LC-MS/MS analysis, we use a combination of ETD, HCD, and CID to obtain as much secondary fragmentation information of the peptides as possible. Through software analysis and manual identification of the secondary spectra of glycosylated peptide fragments, accurate information about the glycosylation sites and their glycoforms of the target protein is obtained.

     

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    Gashash, E. A. et al. J Proteome Res. 2017.

    Figure 1. Glycosylation Sites and Glycoform Analysis Process

     

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    Figure 2. Glycosylation Sites and Glycoform Analysis

     

    Service Advantages

    1. Accurate Analysis of the Glycoforms Corresponding to the Glycosylation Sites in the Target Protein

    2. High Sensitivity and Good Reproducibility

     

    Applications

    1. Identification of the Glycoforms Corresponding to N-glycosylation Sites in Glycosylated Proteins

    2. Identification of the Glycoforms Corresponding to O-glycosylation Sites in Glycosylated Proteins

     

    Sample Submission Requirements

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    Figure 3. Glycosylation Sites and Glycoform Analysis Sample Requirements

     

    1. If you provide tissue samples, please transport on dry ice.

    2. If you provide protein samples, please transport protein extracted from common tissues and cell lysis buffer.

    3. Sample Transport: Please use an ample amount of dry ice and opt for the fastest possible mailing method to minimize sample degradation during transportation.

     

    Deliverables

    1. Experimental Procedures

    2. Mass Spectrometric Parameters

    3. Identification information for protein N-type and O-type glycoforms

    4. Mass Spectrometric Images

    5. Raw Data

     

    Services at MtoZ Biolabs

    1. N-Glycan Modification and Site Analysis Service

    2. O-Glycan Modification and Site Analysis Service

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