Fungi Peptidomics Analysis Service
Fungi peptidomics refers to the systematic identification and quantification of naturally occurring endogenous peptides in fungal samples, typically using high-resolution mass spectrometry. These low-molecular-weight peptides play critical roles in signal transduction, metabolic regulation, and stress response. Their expression profiles can reflect the physiological or pathological state of fungi under specific conditions.
With years of experience in mass spectrometry, MtoZ Biolabs has established a comprehensive Fungi Peptidomics Analysis Service covering sample preparation, peptide enrichment, LC-MS/MS analysis, and bioinformatics interpretation for global academic and industrial clients.
Services at MtoZ Biolabs
The core of fungi peptidomics analysis lies in mass spectrometry, complemented by front-end sample preparation and separation strategies. It enables the identification, quantification, and source protein mapping of endogenous peptides from complex fungal matrices. Our Fungi Peptidomics Analysis Service includes but are not limited to:
1. Extraction and analysis of natural peptides from fungal samples: Low-molecular-weight peptides are extracted from mycelia, fruiting bodies, or fermentation supernatants using molecular weight cutoff filters, solid-phase extraction, or centrifugation-based methods.
2. LC-MS/MS peptide identification: High-resolution mass spectrometry combined with nano-LC is employed to generate confident peptide sequences and associated parameters.
3. Comparative analysis of multiple conditions: Supports comparison across multiple time points or treatment conditions to identify differentially expressed endogenous peptides.
4. Protein mapping and annotation: Peptides are mapped to their putative source proteins via database searching for further functional analysis.
Analysis Workflow
1. Sample Receipt and Quality Control
Each sample is labeled and inspected for integrity, with total protein concentration assessed to ensure analytical suitability.
2. Peptide Extraction and Enrichment
Based on sample type, appropriate strategies such as molecular weight cutoff filtration, SPE, or ultrafiltration are applied to isolate natural endogenous peptides.
3. LC-MS/MS Analysis
Samples are analyzed using high-resolution Orbitrap instruments coupled with nano-LC to acquire peptide m/z values, intensities, and retention times.
4. Database Searching and Quantitative Analysis
Specialized software is used to match peptide spectra against fungal protein databases, enabling identification and quantification.
5. Report Generation
Standardized reports including peptide lists, protein mapping, comparative analyses, and graphical visualization are compiled for interpretation and presentation.
Service Advantages
1. Fungal-Specific Sample Preparation Protocols
We have optimized lysis and extraction workflows to account for the tough cell walls and unique biochemistry of fungi. This ensures compatibility with various sample types such as mycelia, fruiting bodies, and fermentation broth while maximizing the retention of endogenous peptides.
2. Advanced High-Resolution Mass Spectrometry
With multiple Orbitrap instruments (Fusion Lumos, Q Exactive HF) and nano-flow LC systems, we deliver sensitive and wide dynamic range detection, enabling accurate quantification of low-abundance peptides in complex fungal matrices.
3. Transparent Pricing
MtoZ Biolabs adheres to a clear and transparent pricing policy. Before the project begins, we provide a detailed quotation outlining each experimental step, analytical content, and associated costs. No hidden fees. Any changes to the scope will be confirmed with the client before execution, enabling effective budget control and full project oversight.
Applications
Fungi Peptidomics Analysis Service is widely applicable in both basic research and industrial contexts, including but not limited to:
1. Discovery and characterization of natural peptides in medicinal fungi
2. Optimization of industrial fungal strains and metabolic pathway analysis
3. Identification of differential peptides in pathogenic fungi and physiological state monitoring
4. Comparative analysis of peptide profiles in edible fungi and quality assessment
5. Screening of signal peptides in fungal-host interaction studies
6. Characterization and QC of peptide products in fermentation processes
FAQs
Q1: What types of samples are suitable for fungi peptidomics analysis service?
A1: Common samples include fungal mycelial powder, fruiting bodies, fermentation supernatants, and crude lysates. Samples should be intact and free from contamination; lyophilization before submission is recommended.
Q2: What is the typical turnaround time?
A2: Standard turnaround is 3–4 weeks depending on sample quantity and project complexity.
Q3: Can you provide publication-ready reports?
A3: Yes. We provide standardized report formats including annotations and figures suitable for direct inclusion in manuscripts.
Sample Submission Suggestions
| Sample Type | Recommended Amount | Storage Conditions |
| Mycelial Powder | ≥ 50 mg | −80°C frozen storage |
| Fruiting Body Tissue | ≥ 100 mg | Lyophilized preferred |
| Fermentation Supernatant | ≥ 1 mL | −80°C storage, avoid freeze-thaw |
| Crude Lysate/Extract | ≥ 100 µg total protein | Transport on ice or dry ice |
Please contact us for a detailed sample preparation guide to ensure optimal analytical outcomes.
Deliverables
1. Peptide identification list (sequence, m/z, intensity, charge state, etc.)
2. Protein mapping information
3. Differential expression analysis results across groups
4. Graphical visualization (volcano plots, heatmaps, etc.)
5. Standard PDF report
6. Raw MS data (.RAW files)
7. Data processing documentation (if applicable)
Fungi peptidomics is becoming a powerful tool for exploring functional expression in microbial systems. Backed by advanced mass spectrometry platforms and streamlined workflows, MtoZ Biolabs delivers dependable and high-quality Fungi Peptidomics Analysis Service to help researchers uncover peptide-level signatures and gain deeper insights into fungal biology.
Contact us today to receive a customized proposal and quotation.
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