Exosome Tracking Service

Exosomes are widely present in various body fluids, carrying biologically active molecules such as proteins, lipids, and RNAs, and play essential roles in intercellular communication, disease regulation, immune responses, and drug delivery processes. In recent years, with deepening research on exosomes, their functions in basic biology and clinical medicine have gradually become clear. However, the behavior of exosomes in vivo and in vitro remains somewhat complex. Exosome tracking analysis is an analytical technology used to monitor the dynamic distribution, homing characteristics, and uptake pathways of exosomes in vivo or in vitro. It aims to reveal their roles in intercellular communication, targeted delivery, and disease regulation, thereby achieving visual tracking of exosomes across temporal and spatial dimensions.

 

Exosome tracking service is widely applied in oncology, immunology, neuroscience,regenerative medicine, and drug delivery system research. Through precise exosome tracking, researchers can gain deeper insights into the underlying mechanisms of exosomes in tumor metastasis, immune regulation, targeted therapy, and tissue repair, providing critical data support for functional exosome studies, drug development, and clinical translation.

 



Shen, L M. et al. ACS Applied Nano Materials, 2021.

Figure 1. Different Strategies for Exosome Labeling and Imaging.

 

Services at MtoZ Biolabs

Based on a combination of multiple advanced imaging and analytical platforms as well as various labeling methods, the exosome tracking service provided by MtoZ Biolabs enables dynamic tracking and spatial localization analysis of exosomes. This service can offer critical data on the distribution pathways, tissue-homing characteristics, and uptake efficiency of exosomes within cells or animal models, ensuring high-resolution, quantitative, and reproducible imaging results. Thus, it provides strong support for functional validation of exosomes in drug delivery, disease research, and biomarker development.

 

Service Advantages

1. Advanced Platform and Equipment

Our service is supported by advanced equipment such as imaging systems and flow cytometry, establishing a comprehensive exosome tracking service platform. This enables real-time, visual tracking of exosomes both in vivo and in vitro.

 

2. Flexible Labeling Strategies

We offer multiple well-established exosome labeling methods. Our technical team can customize the optimal labeling strategy based on experimental objectives, exosome sources, and downstream analyses, ensuring high signal intensity, low background interference, and excellent labeling stability.

 

3. Experienced Professional Team

We have a highly experienced experimental and project management team, providing one-stop services from experimental design, execution, to data analysis. Additionally, we offer personalized technical recommendations and in-depth result interpretations tailored to your specific project objectives.

 

4. Customized Service

We offer flexible, customized experimental plans tailored to your specific needs, supporting various experimental systems such as cell lines and mouse models, thereby accommodating diverse experimental protocols.

 

Applications

1. Drug Delivery Research

The exosome tracking service can be applied to evaluate the in vivo distribution pathways, targeted homing capabilities, and delivery efficiency of engineered exosomes. This supports the feasibility of developing exosomes as novel nanocarriers for drug delivery.

 

2. Research on Tumor Metastasis and Immune Evasion Mechanisms

By tracking migration trajectories and tissue localization of tumor cell-derived exosomes in vivo, our service reveals their roles in modulating the tumor microenvironment, promoting immune evasion, or facilitating metastasis processes.

 

3. Functional Validation of Stem Cell-Derived Exosomes

The exosome tracking service can be utilized to investigate the enrichment behavior of stem cell-derived exosomes in damaged tissues, analyzing their potential mechanisms in tissue repair and regenerative medicine.

 

4. Inflammation and Immune Response Regulation

By exploring the dynamic behavior of immune cell-derived exosomes at inflammation sites, this service aids in understanding their biological significance in regulating immune signaling and cellular communication.

 

5. Mechanistic Exploration in Disease Models

Our exosome tracking service supports studies on the spatial and temporal distribution of specific exosomes within animal disease models, facilitating dynamic observation and mechanistic hypothesis validation during pathological processes.

 

Case Study

1. Visualization of Exosomes from Mesenchymal Stem Cells in Vivo by Magnetic Resonance Imaging

This study aimed to evaluate the feasibility of visualizing mesenchymal stem cell (MSC)-derived exosomes in vivo using magnetic resonance imaging (MRI). Using a mouse model, the researchers labeled MSC-derived exosomes by co-incubation with superparamagnetic iron oxide nanoparticles (SPIONs). The labeled exosomes were then injected intravenously into mice via the tail vein, and their distribution was tracked using MRI. Results demonstrated that exosomes were successfully labeled by SPIONs, with the labeling process not affecting exosome morphology and function. MRI imaging showed that the labeled exosomes mainly accumulated in the liver and spleen, with the signal intensity peaking at 24 hours post-injection before gradually declining. The study concluded that SPION-labeled exosomes could be noninvasively tracked in vivo by MRI, providing a novel tool for investigating exosome behavior and function in living organisms.

 



Liu, T Q. et al. Magnetic Resonance Imaging, 2020.

Figure 2. Characterization of Exosome–MSC and Exosome–MSC/FTH1.

 

2. In Vivo Visualization of Murine Melanoma Cells B16-derived Exosomes Through Magnetic Resonance Imaging

This study aimed to visualize exosomes derived from murine melanoma B16 cells using magnetic resonance imaging (MRI) to better understand their distribution and dynamic behavior in vivo. Researchers stably introduced an MRI imaging reporter system into B16 cells using lentiviral transduction, and subsequently isolated the labeled exosomes from these cells. In vitro experiments confirmed the exosomes' characteristics, ensuring their morphology and functionality were unaffected by the labeling process. Upon intravenous injection into mice, MRI was used for real-time tracking, revealing that the labeled exosomes primarily accumulated in organs such as the liver and lungs. The study successfully demonstrated the feasibility of visualizing specific cell-derived exosomes in vivo using MRI, providing a novel approach and perspective for future research on exosomes in disease diagnosis and therapy.

 



Liu, T Q. et al. Biochimica et Biophysica Acta (BBA)-General Subjects, 2022.

Figure 3. The Graphical Abstract of the Study.

 

FAQ

Q1: What Sources of Exosomes Can Be Tracked?

A1: We can track exosomes from a wide range of sources, including but not limited to tumor cells, stem cells, and immune cells. Samples can be derived from cell culture supernatants, serum, plasma, urine, and other sources.

 

Q2: Can Tracking Services Be Combined with Other Analyses Such as Functional Validation or Multi-Omics Studies?

A2: Yes. We provide comprehensive services that include post-tracking analyses such as exosome uptake validation in target tissues, protein/miRNA expression profiling, and functional assays, enabling a complete research workflow.

 

Q3: How to Ensure the Reliability of Tracking Results?

A3: We utilize highly sensitive imaging platforms and standardized experimental procedures, including appropriate control and blank groups, to guarantee the scientific validity and reproducibility of experimental data.