Exosome Proteomics: Overview and Workflow

Exosome proteomics profiles proteins in 30–150 nm extracellular vesicles from biofluids and cell culture—supporting liquid biopsy, signaling studies, and biomarker discovery.

Key takeaways

• EV cargo reflects parent-cell state and disease biology.
• Isolation (UC, SEC, immunocapture) sets MS specificity.
• Quantification options include DIA, TMT, and label-free LC-MS/MS.
• Bioinformatics links proteins to pathways and EV databases.





Related services

Exosome proteomics Exosome Proteomics Service Exosome Proteomics Analysis Service Exosome Label-Free Quantitative Proteomics Service LC-MS/MS Exosome Proteomics Service

Quantitative MS DIA Proteomics Analysis Service Label-Free Quantitative Proteomics Service TMT Quantitative Proteomics Service Deep DIA Proteomics Service



FAQ

How pure should EV prep be? Increase stringency (SEC, beads) when serum protein carryover limits IDs.

DIA or label-free? DIA for reproducible cohorts; label-free for flexible designs.

Conclusion

Exosome proteomics connects vesicle biology to measurable protein signatures in accessible fluids.