Electron Microscopy-Based Mitochondrial Phenotype Analysis Service
- Disease Mechanism Research: By observing structural abnormalities in mitochondria, this service helps reveal the molecular mechanisms of diseases.
- Drug Screening and Mechanism of Action: By examining the morphological changes in mitochondria before and after drug treatment, this service provides data support for drug screening and mechanism of action studies.
- Cellular Stress Response and Oxidative Stress Research: Observing mitochondrial morphological changes under stress conditions, this service helps study how cells respond to external stimuli such as drugs, toxic substances, or hypoxia.
- Mitochondrial Function and Quality Control: By observing mitochondrial morphological changes under various physiological or pathological conditions, this service aids in the study of mitochondrial quality control and maintenance mechanisms.
Electron Microscopy-Based Mitochondrial Phenotype Analysis Service is a high-resolution imaging and analysis service that utilizes transmission electron microscopy (TEM) technology to study the ultrastructure of mitochondria in cell or tissue samples. This service can help researchers gain deep insights into the functional state of mitochondria and their role in health and disease by observing the morphology, size, inner and outer membrane structures, and interactions with other organelles.
Mitochondria are the cell’s energy factories, responsible for energy production, regulating cell metabolism, and maintaining cellular homeostasis. The health of mitochondria is closely linked to many physiological processes, including cellular energy metabolism, cell death, and oxidative stress responses. Damage or dysfunction of mitochondria is directly associated with various diseases. Mitochondrial dysfunction is often accompanied by changes in morphology, which serve as early indicators of disease onset and progression.
Electron microscopy (EM), as a high-resolution imaging technology, can clearly observe ultrastructural changes in mitochondria, including the morphology of mitochondrial membranes, cristae structure, and interactions with other organelles. Using electron microscopy, researchers can better understand the changes in mitochondrial function in health and disease, providing crucial structural evidence for disease mechanism studies, drug screening, and clinical applications.
Leveraging a transmission electron microscopy platform, MtoZ Biolabs offers Electron Microscopy-Based Mitochondrial Phenotype Analysis Service to accurately capture the morphology of inner and outer membranes, cristae structure, interactions between mitochondria and other organelles, and changes in mitochondrial morphology under different physiological and pathological states, providing key insights into mitochondrial health, functional impairment, or autophagy processes.
Kamogashira T. et al. npj Aging Mech Dis. 2017.
Figure 1. Change in mitochondrial ultrastructures in H2O2-exposed cells.
Analysis Workflow
General workflow of Electron Microscopy-Based Mitochondrial Phenotype Analysis Service:
1. Sample Preparation and Fixation
Cells or tissue samples are treated with fixatives to preserve the mitochondrial structure.
2. Ultrathin Sectioning
The fixed samples are sliced into ultrathin sections suitable for transmission electron microscopy observation.
3. Transmission Electron Microscopy Imaging
High-resolution morphological images of mitochondria are obtained using a transmission electron microscope.
4. Morphological Analysis and Quantification
The images are analyzed to measure parameters such as mitochondrial shape, size, and the number of cristae.
5. Data Processing and Reporting
Statistical analysis and interpretation of the data are performed, and an analytical report is generated.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Mitochondrial Phenotype Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Resolution Imaging: Ultra-high resolution imaging capabilities provide nanometer-level images, ensuring precise capture of mitochondrial microscopic changes.
4. Professional Data Analysis and Interpretation: With advanced data processing platforms and a team of expert biologists, we help better understand the changes in mitochondrial phenotypes and their biological significance.
Sample Submission Suggestions
Sample Types: Both cell and tissue samples are acceptable.
Feel free to contact us for detailed and targeted sample preparation recommendations before submitting your samples.
Applications
Applications of Electron Microscopy-Based Mitochondrial Phenotype Analysis Service:
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. High-Resolution TEM Images
4. Quantitative Morphological Analysis Report
5. Biological Interpretation
Related Services
Mitochondrial Phenotype Analysis Service
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