DNA/RNA Drug Higher-Order Structure Analysis Service
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DNA/RNA Drug Higher-Order Structure Analysis Service is a service dedicated to the systematic study and characterization of the three-dimensional conformations formed by nucleic acid drugs under near-physiological conditions. The goal of this service is to confirm whether nucleic acid drugs maintain their intended spatial folding, evaluate their stability, polymorphism, and functional relevance, and provide reliable data support for drug development, delivery system optimization, and regulatory submission.
In the development of nucleic acid drugs such as antisense oligonucleotides, siRNA, mRNA vaccines, and aptamers, the molecular sequence is only the first step in determining function. DNA and RNA further fold in vivo and under near-physiological conditions to form complex higher-order structures, including three-dimensional folding patterns, long-range interactions, supercoiled states, and multimeric conformations. These structures directly affect drug stability, delivery efficiency, immunogenicity, and binding specificity with targets. Abnormal or unstable higher-order structures may reduce efficacy or even pose safety risks, making systematic characterization of higher-order structures an essential part of drug development, quality control, and regulatory compliance.
Services at MtoZ Biolabs
By integrating high-resolution mass spectrometry, nuclear magnetic resonance (NMR), cryo-electron microscopy (Cryo-EM), X-ray crystallography, and other advanced techniques, MtoZ Biolabs provides DNA/RNA Drug Higher-Order Structure Analysis Service to comprehensively characterize the three-dimensional folding patterns and spatial conformations of nucleic acid drugs under near-physiological conditions. Our service covers not only global conformation detection and thermal stability analysis but also reveals the impact of long-range interactions and modifications on higher-order structures. Through the integration of experimental methods and computational simulations, MtoZ Biolabs delivers multi-level structural characterization from overall to atomic resolution, supporting drug design optimization, quality consistency evaluation, and regulatory submission.
Kloczewiak M. et al. Mol Pharm. 2022.
Figure 1. Representations of RNA higher-order structure showing how secondary structures formed from complementary base pairing fold into 3-dimensional tertiary structures.
Analysis Workflow
The general workflow of DNA/RNA Drug Higher-Order Structure Analysis Service is as follows:
1. Sample Preparation and Quality Assessment
Ensure DNA/RNA samples have high purity and integrity to avoid results being affected by degradation or modification loss.
2. Selection of Analytical Methods
Choose appropriate platforms based on research objectives, such as NMR and crystallography for high-resolution structural analysis, and CD and MS for overall conformation and modification detection.
3. Data Acquisition
Collect spectroscopic, mass spectrometric, or imaging data using the selected techniques.
4. Data Analysis and Interpretation
Process data with specialized software and compare results with theoretical models or known structures.
5. Comprehensive Report Generation
Deliver a complete report including experimental results, predicted models, and functional analysis.
Service Advantages
Advanced Analysis Platform: MtoZ Biolabs established an advanced DNA/RNA Drug Higher-Order Structure Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all DNA/RNA Drug Higher-Order Structure Analysis data, providing clients with a comprehensive data report.
Customized Solutions: Provide personalized experimental design based on the client’s research objectives.
Sample Submission Suggestions
Multiple types of DNA and RNA drugs are acceptable.
Store at low temperature (-20°C or below), ship on dry ice, and avoid repeated freeze and thaw cycles.
It is recommended to contact the MtoZ Biolabs technical team prior to sample submission to obtain detailed and tailored guidelines for sample preparation and submission.
Applications
Antisense and RNA Interference Drug Research: Analyze how higher-order structures affect target binding efficiency, nucleic acid stability, and degradation pathways to guide potency optimization.
Drug Delivery System Evaluation: Study the conformational stability of DNA/RNA drugs in different delivery carrier environments to support delivery strategy optimization.
Vaccine and Virology Research: Reveal the folding characteristics of viral genomes and mRNA vaccines to provide a basis for vaccine design and immunogenicity prediction.
Quality Control and Regulatory Submission: Ensure drugs maintain their intended conformations during production and storage, and provide structural validation data required for regulatory support.
FAQ
Q1: What Resolution Can Be Achieved by Different Methods?
A1: NMR and X-ray crystallography can resolve structures at the atomic level, Cryo-EM is suitable for near-atomic resolution imaging of macromolecules or complexes, AFM provides nanoscale surface topology information, while CD and mass spectrometry are suitable for detecting overall conformation and polymorphism.
Q2: How Do Chemical Modifications Affect Higher-Order Structures?
A2: Common modifications such as 2'-O-methylation, PS bonds, and thiolation may alter folding stability or spatial arrangement. By combining mass spectrometry and NMR with thermodynamic analysis, the specific impact of modifications on overall conformation can be revealed.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
DNA/RNA Drug Structural Characterization Service
DNA/RNA Drug Sequence Confirmation Service
DNA/RNA Drug Secondary Structures Analysis Service
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