DIA-MS Ubiquitinome Analysis Services
- Species flexibility: Human, mouse, rat, plant, and microbial samples.
- Compatibility with complex matrices: Tissue lysates, cell lines, serum, and FFPE samples.
- Comparative ubiquitinomics: Time-series, dose-response, and disease vs. control studies.
Ubiquitination is a pivotal post-translational modification (PTM) in which ubiquitin, a small regulatory protein, covalently attaches to substrate proteins via lysine residues. This dynamic process regulates critical cellular functions, including protein degradation via the ubiquitin-proteasome system (UPS), DNA repair, signal transduction, and immune response. Dysregulation of ubiquitination is closely linked to severe pathologies such as neurodegenerative diseases (e.g., Alzheimer´s, Parkinson´s), cancer progression, and autoimmune disorders.
Damgaard, RB. Cell Death Differ. 2021.
Figure 1. Ubiquitin Signalling in Cell Biology and Disease
Studying ubiquitinated proteins presents significant challenges due to their low abundance, transient nature, and complex regulatory networks. Traditional methods like immunoassays and Western blotting lack the throughput and specificity for large-scale analysis. Mass spectrometry (MS)-based proteomics has emerged as the gold standard, enabling systematic identification and quantification of ubiquitination sites. Conventional data-dependent acquisition (DDA-MS) often suffers from stochastic sampling bias, leading to poor reproducibility in detecting low-abundance ubiquitinated peptides. DIA-MS (Data-Independent Acquisition) overcomes these limitations by fragmenting all precursor ions within specified m/z windows, ensuring consistent coverage and high reproducibility. This technology is particularly advantageous for ubiquitinome studies, offering higher sensitivity for rare modifications, improved quantitation accuracy across diverse samples, and enhanced capability to resolve site-specific ubiquitination dynamics.
Service at MtoZ Biolabs
At MtoZ Biolabs, we combine cutting-edge DIA-MS technology with advanced bioinformatics to deliver unparalleled DIA-MS ubiquitinome analysis services. Our services cater to researchers aiming to unravel the complexity of ubiquitin signaling in health and disease.
Key Features of Our Service
1. Comprehensive Workflow
Sample Preparation: Optimized enrichment protocols using anti-ubiquitin antibodies or ubiquitin remnant motif affinity strategies.
DIA-MS Analysis: High-resolution LC-MS/MS platforms (e.g., Orbitrap Exploris 480, timsTOF Pro) ensure deep proteome coverage.
Bioinformatics: Customized pipelines for ubiquitination site localization, pathway enrichment, and interactive visualization.
Figure 2. The Workflow of Ubiquitome Analysis
2. Tailored Solutions
Why Choose MtoZ Biolabs?
✔️ Unmatched Sensitivity & Depth：We Detect over 10,000 ubiquitination sites per sample, even in low-input clinical specimens.
✔️ DIA-MS Technical Superiority: By leveraging the latest DIA-MS technology, we ensure high-quality data acquisition, overcoming the limitations of traditional data-dependent acquisition (DDA-MS) in analyzing low-abundance peptides. This results in higher sensitivity and accuracy.
✔️ High Throughput for Large-Scale Studies: We offer high-throughput analysis services suitable for large-scale sample analysis. Whether generating multi-omics data or efficiently handling complex experimental conditions, MtoZ Biolabs can meet your needs.
Applications
Our DIA-MS ubiquitinome profiling services empower researchers across diverse fields to decode ubiquitin-mediated regulatory mechanisms and drive innovation in both basic and translational research. Key applications include:
1. Disease Mechanism Research
2. Drug Development & Precision Medicine
3. Agricultural and Microbial Research
4. Systems Biology
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Relevant Liquid Chromatography and Mass Spectrometry Parameters
4. The Detailed Information of Ubiquitinome Analysis
5. Mass Spectrometry Image
6. Raw Data
Whether unraveling disease mechanisms, accelerating drug discovery, or exploring evolutionary adaptations, our DIA-MS ubiquitinome analysis services deliver actionable insights with unmatched precision. Partner with us to transform your research into breakthroughs. Contact us today to discuss your project and request a custom proposal!
How to order?
