Custom ADC Synthesis Service

Antibody-drug conjugates (ADCs) are a class of targeted therapeutics that combine monoclonal antibodies with cytotoxic small molecules via specialized linkers. With their high specificity, potent cytotoxicity, and proven efficacy, ADCs have demonstrated broad clinical potential, especially in oncology. As bioconjugation and antibody engineering technologies continue to advance, ADC development has moved from basic research into clinical translation, placing increased demands on precise conjugation control, structural characterization, and product consistency.

 

As a specialized CRO in custom chemical synthesis, MtoZ Biolabs offers a one-stop Custom ADC Synthesis Service that covers the entire process—from antibody preparation and linker-payload screening to conjugation strategy design, structural confirmation, and quality assessment—supporting clients in the efficient advancement of diverse ADC development projects.

 

Services at MtoZ Biolabs

An ideal ADC should exhibit uniform structure, high stability, and controlled drug-to-antibody ratio (DAR) to ensure predictable pharmacokinetics, safety, and efficacy. However, traditional ADC synthesis processes often face challenges such as heterogeneous conjugation sites, isomeric mixtures, nonspecific modifications, and unstable DAR distribution, which hinder product consistency and development efficiency.

 

To address these issues, MtoZ Biolabs has integrated tunable site-selective conjugation strategies and small-molecule payload screening workflows into its ADC synthesis platform. These are combined with real-time mass spectrometry monitoring to significantly improve conjugation precision and DAR consistency while minimizing off-target modifications and byproduct formation. This approach enhances structural control and reproducibility from the outset of ADC construction.

 

Our custom ADC synthesis service covers several key modules, including but not limited to:

1. Linker–Payload Design: Selection of optimal linker–payload combinations based on the biological target and therapeutic context, balancing release mechanism, stability, and pharmacokinetics.

2. Conjugation Strategy Optimization: Implementation of lysine-, cysteine-, site-specific-, or click chemistry-based conjugation strategies to improve DAR control and structural uniformity.

3. Purification and Characterization: Comprehensive analysis using SEC, HIC, CE-SDS, ESI-MS, and high-resolution Orbitrap mass spectrometry to evaluate DAR, aggregate content, and payload attachment sites.

 

Analysis Workflow

 



 

This workflow is standardized but can be flexibly adjusted according to project-specific requirements.

 

Service Advantages

1. Versatile Conjugation Strategies

MtoZ Biolabs has established a modular ADC development platform compatible with a range of conjugation methods, including lysine, cysteine, site-specific, and click chemistry. We tailor conjugation approaches based on antibody structure, payload functionality, and target DAR to optimize construct efficiency and consistency—especially suitable for early-stage screening and process evaluation.

 

2. Precise DAR and Structural Characterization

Utilizing high-resolution Orbitrap mass spectrometry and nanoLC systems, we provide accurate assessment of key ADC quality attributes such as DAR distribution, main peak purity, residual free drug, and isomer resolution. This enables reliable quality control and informed decision-making.

 

3. Extensive Payload Handling Expertise

Our team is experienced in handling common payloads including MMAE, DM1, SN-38, and PBDs, and is familiar with their chemical behavior under different linker strategies. This minimizes risks such as hydrolysis or unwanted isomer formation and ensures antibody integrity, reducing development timelines and trial-and-error costs.

 

4. Rigorous Quality Control

Each project is executed under SOP-based workflows covering reagent preparation, conjugation conditions, purification, and analytical testing. Comprehensive analysis reports are provided, including raw data plots, peak ratios, sample details, and method descriptions to support regulatory documentation and traceability.

 

5. Flexible Collaboration Models

We offer modular service options—from standalone DAR analysis and structure confirmation to full-scale ADC development workflows—accommodating various development stages and budget constraints. Whether for early-stage screening or late-stage process validation, we provide tailored technical support and data interpretation for efficient collaboration.

 

Applications

1. Screening and construction of ADC candidates

2. Evaluation of novel payload/linker combinations

3. Feasibility testing of antibody engineering constructs

4. Quality control and DAR analysis for process consistency

5. High-throughput bioconjugate studies and platform development

 

FAQ

Q1: Can clients provide their own antibodies or payloads?

A: Yes. Clients may submit antibodies (liquid or lyophilized), payloads, or linkers. We will perform conjugation as specified.

 

Q2: What conjugation strategies are supported?

A: We support lysine conjugation, cysteine conjugation, click chemistry, and enzyme-mediated site-specific conjugation, selected according to molecular structure and project goals.

 

Q3: What is the typical turnaround time?

A: Standard turnaround is 2–4 weeks depending on sample status and requested services. Expedited options are available upon request.

 

Q4: Can regulatory-compliant reports be provided?

A: Yes. Our reports are suitable for GLP research support and IND-stage regulatory submission requirements.

 

Sample Submission Suggestions

1. Antibody: ≥1 mg recommended; buffer should be PBS or Tris (pH 6.5–7.5) without azide, glycerol, or surfactants.

2. Payload/Linker: Please provide molecular weight, solubility, and storage conditions.

3. Storage & Shipping: Store at -80°C; ship with dry ice.

4. Additional Info: Provide antibody sequence or functional region details (e.g., Fc mutations) to support site-specific conjugation strategy design.

 

Deliverables

1. ADC Sample: Delivered in lyophilized or liquid form, labeled with key specifications such as concentration and buffer composition.

2. Analytical Report: Includes DAR data, purity assessment, LC-MS spectra, chromatographic profiles, and preliminary structural confirmation.

3. Experimental Summary: Method overview, parameter settings, and conjugation efficiency for documentation or comparison.

4. Storage and Handling Guidelines: Custom recommendations for storage, reconstitution, and usage based on product characteristics.

 

ADC development is a complex, multidisciplinary process involving chemical modification, protein engineering, and structural validation. MtoZ Biolabs is committed to delivering standardized yet customizable Custom ADC Synthesis Services that span from early-stage design to DAR-based quality control. If you are planning an ADC development project or require technical collaboration, we welcome you to contact us for customized proposals and expert consultation. We are here to support your innovation with reliable, professional, and efficient solutions.