Cryo-EM Survey Standard Evaluation Service
- Proteins and complexes: Assess particle monodispersity and aggregation status
- Membrane proteins and nanodiscs: Evaluate orientation bias and background noise
- Viruses and VLPs: Examine morphological integrity and particle uniformity
- Vesicles and flexible nanostructures: Optimize vitrification for non-rigid assemblies
- RNA and RNPs: Characterize conformational consistency and particle quality
Cryo-EM Survey Standard Evaluation Service is a streamlined and standardized pre-analysis designed to assess sample suitability for cryo-electron microscopy (Cryo-EM) structural studies. Through negative-stain screening and preliminary cryo-imaging, this service evaluates molecular features, imaging quality, and sample handling complexity—serving as an essential step before committing to high-resolution data acquisition.
As a transformative technique in structural biology, Cryo-EM enables 3D visualization of macromolecules without crystallization. However, factors like sample heterogeneity, particle distribution, and ice thickness can significantly impact imaging outcomes. To mitigate these risks, MtoZ Biolabs offers expert-driven Cryo-EM Survey Standard Evaluation Service using a well-established platform and robust workflows, empowering researchers to make informed decisions and accelerate structural discovery with confidence.
Services at MtoZ Biolabs
MtoZ Biolabs offers the Cryo-EM Survey Standard Evaluation Service with a fully standardized workflow covering sample assessment, grid screening, and image pre-processing—designed to help researchers rapidly and reliably kickstart their structural studies.
1. Negative-Stain Screening
Rapid evaluation of particle morphology, distribution, and aggregation tendencies.
2. Cryo-Grid Preparation
Freeze grids under various conditions to assess sample behavior upon vitrification.
3. Preliminary Imaging
Capture representative micrographs using cryo-electron microscopy.
4. 2D Classification and Image Alignment
Analyze particle orientation, uniformity, and data usability through advanced processing.
5. Technical Assessment Report
Deliver a detailed evaluation of imaging performance, data quality, and recommendations for downstream Cryo-EM studies.
This Cryo-EM Survey Standard Evaluation Service acts as the launchpad for structural biology projects, offering vital insights into sample quality and informing strategies for high-resolution imaging and model reconstruction.
Service Advantages
· Broad Sample Compatibility
Supports protein complexes, membrane proteins, viruses, vesicles, and other biological nanostructures.
· Fully Standardized Workflow
End-to-end SOP from grid screening to preprocessing ensures consistency and reproducibility across samples.
· Minimal Sample Input Required
Advanced micro-preparation and automation technologies maximize the value of limited or precious biological samples.
· Direct Transition to High-Resolution Analysis
Seamless handoff to MtoZ Biolabs’ Cryo-EM data acquisition and 3D reconstruction services saves time and resources.
· One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
Applications
The Cryo-EM Survey Standard Evaluation Service is applicable to a wide range of structural and drug discovery research contexts:
FAQ
Q1: Can the data from the survey be used for 3D reconstruction?
Yes. If evaluation results are favorable, the acquired micrographs and 2D classification can directly support downstream 3D structure reconstruction, eliminating the need for repeat acquisition.
Q2: How much sample is needed for evaluation?
We recommend submitting 50–100 µL of sample. Final volume requirements depend on the sample type and concentration.
Backed by a state-of-the-art Cryo-EM platform and rigorous SOPs, MtoZ Biolabs delivers high-quality, risk-mitigating structural evaluation services tailored to the needs of academic and pharmaceutical research. Our Cryo-EM Survey Standard Evaluation Service lays the groundwork for successful 3D structural studies—enabling data-driven optimization from the very first step. Free project evaluation, welcome to learn more details!
How to order?
