Protein Circular Dichroism Analysis Service

Proteins exhibit asymmetric secondary structures such as α-helices, β-sheets, β-turns, and others, leading to differential absorption of left and right circularly polarized light. Upon transmission through these proteins, the circularly polarized light becomes elliptically polarized, a phenomenon known as protein circular dichroism (CD). CD Spectroscopy is an essential technique for analyzing the secondary and tertiary structures of optically active molecules (e.g., proteins, DNA) in solution. It is widely utilized in studying the structure-function relationship and interactions of macromolecules.

 

MtoZ Biolabs offers professional CD services for analyzing and determining the spatial configurations of proteins.

 

Service Advantages

1. Shorter, Simpler, and More Efficient Measurement Procedures

2. Requires Minimal Sample Volume and Can be Performed in Dilute Solutions

3. No Limitations Regarding Molecular Weight or Size

4. Highly Sensitive to Changes in Secondary and Tertiary Structures, Capable of Detecting Subtle Alterations

 



Norma, J. G. et al. Nat. Protoc. 2006.

Figure 1. CD Spectroscopy Illustrates the Representative Secondary Structures of Peptides and Proteins

 

Sample Submission Requirements

Protein sample concentration should exceed 0.5 mg/mL, with purity exceeding 90%. The minimum quantity of solid protein should not be less than 200 μg.

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with a detailed technical information, including:

1. Experimental Procedures

2. Relevant Data