Protein Secondary Structure Analysis Service

    Protein secondary structure analysis refers to the study of local spatial structures between continuous amino acid residues in proteins, mainly including α-helix, β-sheet, and others. Understanding protein secondary structures helps reveal their folding mechanisms, stability, function, and interactions with other biomolecules, providing important information for studying the biological functions and regulatory mechanisms of proteins. The principles of protein secondary structure analysis mainly rely on the specific responses of different structural elements (such as α-helix and β-sheet) to signals like light and magnetism. Common methods include circular dichroism (CD), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and X-ray crystallography.

     

    MtoZ Biolabs offers a one-stop protein secondary structure analysis service using JASCO circular dichroism spectrometer, professionally determining the spatial configuration of proteins, including α-helix, β-sheet, and random coils, etc. Through the proportion and distribution information of protein sequence's secondary structure, the composition and changes of protein secondary structure are accurately revealed.

     

    Analysis Workflow

    1. Sample Preparation

    Collect purified protein samples, remove impurities, adjust protein concentration and buffer to meet subsequent analysis requirements.

     

    2. Spectrometer Calibration

    Use a set of standard samples to calibrate the spectrometer, ensuring accurate measurement of the protein sample's CD spectra.

     

    3. Spectra Collection

    Place the protein sample in an optical cuvette and measure and collect CD spectra at different wavelengths (typically between 190-250 nm).

     

    4. Data Processing and Analysis

    Use professional software to analyze the measured CD spectra and convert them into proportions of protein secondary structures.

     

    5. Results Interpretation

    Interpret the protein's secondary structures based on the analysis results, assessing its folding degree, stability, and structural features.

     

    Applications

    1. Protein Structure and Function Research

    Analyzing protein secondary structures can reveal functional and structural characteristics of specific protein families, aiding in understanding the mechanisms of protein function and their relationship with structure.

     

    2. Protein Folding Research

    Secondary structure analysis helps understand key intermediates in the protein folding process, thus revealing the folding mechanisms of proteins.

     

    3. Protein Interaction Research

    Analyzing protein secondary structures can enhance understanding of protein interaction mechanisms, providing a basis for biological processes and signal transduction studies.

     

    4. Drug Design and Screening

    Analyzing protein secondary structures can provide important information on the structural characteristics of drug targets, assisting researchers in designing and screening more specific and efficient drugs.

     

    Deliverables

    In the technical report, MtoZ Biolabs will provide you with a detailed technical information, including:

    1. Experimental Procedures

    2. Relevant Mass Spectrometry Parameters

    3. Detailed Information on Protein Secondary Structure Analysis

    4. Mass Spectrometry Images

    5. Raw Data

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