Chemoproteomics: Principles, Workflow, and Applications

Workflow: design selective probes → label live lysates → enrich tagged proteins → LC-MS/MS → map targets and networks.

Key takeaways

• ABPP captures active enzyme pockets.
• Target ID maps drug binders.
• Controls reduce non-specific labeling.
• MS delivers sensitivity at proteome scale.







Related services

Chemoproteomics Chemical Proteomics Analysis Service Activity-Based Protein Profiling (ABPP) Service Target Identification Service Covalent Proteomics Service

MS identification support Drug Target Identification Service Protein Identification by LC-MS/MS Service Shotgun Proteomics Qualitative Proteomics Analysis Service

FAQ

How is chemoproteomics different from standard proteomics? It prioritizes chemical engagement and activity, not abundance alone.

What controls are needed? Competition with free inhibitor and minus-probe controls.

Conclusion

Chemoproteomics reveals functional protein states liganded by small molecules.