Can WB Membranes Be Used for Mass Spectrometry?

Yes, proteins immobilized on Western Blot (WB) membranes can be subjected to mass spectrometry analysis. During the Western Blot procedure, proteins are transferred onto PVDF or nitrocellulose membranes. These protein bands can subsequently be excised from the membrane, enzymatically digested, and analyzed by mass spectrometry to identify the proteins or to perform further characterization.