Blood/Plasma/Serum Proteomics Solutions
- Data-Dependent Acquisition (DDA): Suitable for protein identification, providing high-quality spectral data.
- Data-Independent Acquisition (DIA): Optimized for large-scale quantitative proteomics, ensuring high reproducibility and comprehensive data coverage.
- TMT/iTRAQ Labeling: Ideal for multiplex quantitative analysis, enhancing the sensitivity of comparative proteomics studies.
Blood/Plasma/Serum Proteomics Solutions is an advanced mass spectrometry-based proteomics platform tailored for the comprehensive analysis of blood, plasma, and serum samples. Blood and its derivatives contain a diverse range of proteins that reflect both physiological and pathological states, making blood proteomics a crucial tool for biomarker discovery, early disease diagnosis, personalized medicine, drug development, and precision medicine.
However, plasma and serum proteomics present significant analytical challenges due to their wide dynamic range, where high-abundance proteins (e.g., albumin and immunoglobulins) dominate the total protein content, while low-abundance proteins, which are often key indicators of disease, are more difficult to detect. Overcoming this limitation requires efficient separation, enrichment, and quantification strategies.
MtoZ Biolabs offers high-throughput, sensitive, and precise Blood/Plasma/Serum Proteomics Solutions by leveraging high-resolution mass spectrometry (e.g., LC-MS/MS) and integrating advanced techniques such as protein enrichment, high-abundance protein depletion, data-independent acquisition (DIA), and labeled quantification (TMT/iTRAQ). These technologies empower researchers to elucidate disease mechanisms, identify biomarkers, and drive advancements in personalized medicine.
Services at MtoZ Biolabs
MtoZ Biolabs’ Blood/Plasma/Serum Proteomics Solutions employs liquid chromatography-tandem mass spectrometry (LC-MS/MS) alongside multiple protein pretreatment and data acquisition strategies to ensure high-efficiency protein identification and accurate quantification.
· Sample Preparation: To address the complexity of blood, plasma, and serum proteomes, high-abundance protein depletion techniques (e.g., immunoaffinity columns and magnetic bead enrichment) are applied to reduce interference from albumin and IgG, thereby enhancing the detection of low-abundance proteins.
· Protein Digestion and Peptide Separation: Proteins are digested using enzymatic cleavage (e.g., trypsin, Lys-C) and separated via nano-flow liquid chromatography (Nano-LC) to improve peptide resolution.
· Data Acquisition Modes:
These strategies ensure deep proteome coverage, precise quantification, and robust biological interpretation, laying a solid foundation for downstream bioinformatics analysis.
Figure 1. Blood/Plasma/Serum Proteomics Workflow
Why Choose MtoZ Biolabs?
MtoZ Biolabs’ Blood/Plasma/Serum Proteomics Solutions offers several key advantages:
1. High-Sensitivity Low-Abundance Protein Detection
Optimized depletion strategies improve the detection of low-abundance proteins, enabling biomarker identification.
2. Accurate Quantification & High-Throughput Analysis
Advanced quantification techniques (DIA, TMT/iTRAQ) allow high-precision, high-coverage protein quantification.
3. Comprehensive Data Interpretation & Biological Insights
Supports pathway enrichment analysis and protein interaction network construction, providing multi-level molecular insights.
4. Customized Research Solutions
Tailored data analysis and experimental strategies to accommodate diverse research needs.
Applications
· Biomarker Discovery: Identifying disease-associated biomarkers for cancer, cardiovascular diseases, and autoimmune disorders through differential proteomics.
· Personalized Medicine: Integrating proteomics data to assist in the development of precision diagnostics and therapeutic strategies.
· Drug Mechanism & Pharmacoproteomics: Evaluating drug effects on blood proteomes to optimize drug candidate selection.
· Immunoproteomics & Disease Mechanism Research: Investigating interactions between blood proteins and the immune system to uncover mechanisms of autoimmune diseases and infections.
Case Study
Case 1: Mass Spectrometry-Based Blood Plasma/Serum Proteomics for Biomarker Detection
This study employed targeted proteomics analysis using mass spectrometry to detect cell lysis biomarkers. By integrating glycan-removal techniques, the complexity of plasma/serum samples was reduced, significantly improving biomarker detection sensitivity. This approach allowed for selective enrichment of non-glycosylated proteins, enhancing the accuracy and precision of mass spectrometry-based quantification. Our Blood/Plasma/Serum Proteomics Solutions provides state-of-the-art mass spectrometry and data analysis tools, enabling researchers to accurately quantify blood proteomes and advance biomarker discovery.
Maes, P. et al. Talanta. 2017.
Case 2: Extracellular Vesicle Proteomics from Plasma and Serum Unveils Potential Biomarkers
A proteomic study of extracellular vesicles (EVs) isolated from plasma and serum revealed tissue-specific proteins in healthy individuals. Using affinity capture methods, EVs were isolated from six healthy subjects, followed by high-resolution proteomics analysis, which successfully identified over 4,000 proteins. This dataset provides valuable insights for biomarker discovery, demonstrating the potential of EV proteomics in diagnostic tool development. This study underscores the relevance of Blood/Plasma/Serum Proteomics Solutions in precision medicine and biomarker research.
Muraoka, S. et al. IScience. 2022.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs’ Blood/Plasma/Serum Proteomics Solutions leverages high-resolution mass spectrometry, protein enrichment, depletion strategies, and precise quantification techniques to provide high-quality proteomics solutions for disease research, drug development, and precision medicine.
Whether you are identifying biomarkers, exploring disease mechanisms, or advancing personalized medicine, our team offers cutting-edge experimental and bioinformatics support.
Contact us today to receive a customized research plan and expert technical support!
How to order?
