Bioactive Peptides Analysis Service

Bioactive peptides are short chains of 2–50 amino acids linked by peptide bonds, typically released through enzymatic hydrolysis, fermentation, or digestive processes of natural proteins. Despite their simple structures, these peptides exhibit a wide range of important physiological regulatory functions both in vitro and in vivo, including antihypertensive, antioxidant, antimicrobial, immunomodulatory, and neuroprotective activities. With the rapid development of biopharmaceuticals, functional foods, and novel therapeutic strategies, bioactive peptides have become foundational components in the design and screening of peptide-based drugs.

 

As a CRO specialized in proteomics and peptide analytics, MtoZ Biolabs offers end-to-end bioactive peptides analysis service covering bioactive peptide screening, identification, activity prediction, and mechanism modeling. Supported by advanced mass spectrometry platforms and high-throughput capabilities, we help academic and industrial clients efficiently advance their research and drug development projects.

 

Services at MtoZ Biolabs

MtoZ Biolabs has established a systematic bioactive peptide analysis pipeline based on LC-MS/MS and a suite of bioinformatics tools and functional assessment models. Our bioactive peptides analysis service includes:

 

1. Peptide Extraction and Enrichment

We process enzymatic hydrolysates, fermentation broths, or synthetic peptide mixtures using ultrafiltration, solid-phase extraction (SPE), and C18 materials to purify and concentrate short peptides, enhancing detection sensitivity and data quality.

 

2. Peptide Identification

Utilizing high-resolution LC-MS/MS systems, we perform peptide sequencing through database search and de novo sequencing, supporting both natural and synthetic peptide profiling.

 

3. Bioactivity Prediction and Scoring

We apply bioinformatics tools (e.g., PeptideRanker, antimicrobial peptide predictors) to evaluate the potential bioactivity of identified peptides, prioritizing candidates with antihypertensive, antimicrobial, antioxidant, or immunoregulatory properties.

 

4. Molecular Docking Simulation

Molecular docking is used to simulate peptide–target protein interactions, predict binding sites and affinity, and support mechanism-of-action studies or rational design of optimized peptide variants.

 

Analysis Workflow

 



 

Service Advantages

1. Multi-platform Integration

We integrate Orbitrap and Q-TOF systems to handle various sample types and improve detection of low-abundance peptides, ensuring comprehensive peptide profiling in complex matrices.

 

2. Combined Function Prediction and Structural Analysis

Our service integrates sequence-based bioactivity scoring with target docking and mechanism modeling, providing theoretical support for candidate peptide validation.

 

3. Broad Application Flexibility

Whether screening functional peptides from food-derived proteins or verifying synthetic peptides, we tailor strategies and data interpretation models to support both discovery and applied research.

 

4. Standardized Workflows

We follow strict SOPs from sample receipt to data delivery, ensuring high-quality, reproducible results suitable for scientific publication or regulatory submission.

 

5. Extended Analysis Support

Beyond core services, we offer optional analyses such as peptide stability testing, metabolic pathway prediction, and PK parameter estimation, providing data to support translational research needs.

 

Applications

1. Identification and evaluation of bioactive peptides in functional food development

2. Early-stage development and sequence optimization of novel peptide therapeutics

3. Screening of antimicrobial, antioxidant, and immunoregulatory peptides

4. Mining of functional peptides from food, marine, and animal protein sources

5. R&D support for peptide-based nutraceuticals and health supplements

 

FAQ

Q1: Does the service include experimental validation of activity?

A: Our standard service focuses on prediction and analysis. For in vitro activity validation, please contact us to discuss a customized solution.

 

Q2: What is the typical turnaround time?

A: Standard turnaround is 2–3 weeks. More complex or customized analyses may require additional time.

 

Sample Submission Suggestions

1. Recommended sample amount: ≥100 μL for liquids; ≥100 μg for lyophilized powder

2. Acceptable sample types: enzymatic hydrolysates, fermentation products, natural extracts, peptide mixtures, synthetic peptide powders

3. Storage conditions: store liquid samples at -80°C; store lyophilized samples in a dry, dark environment

4. Required information: sample information form and any specific analysis goals (if available)

 

If you are unsure about your sample type or requirements, please contact us in advance for detailed submission guidelines.

 

Deliverables

1. Raw LC-MS/MS data files

2. Identified peptide list with sequences and associated confidence scores

3. Bioactivity prediction results and functional categorization

4. Molecular docking models and interaction summaries (if applicable)

5. Customized data analysis report with figures, tables, and interpretation

 

Bioactive peptides represent a key molecular link between natural proteins and targeted therapeutic applications. MtoZ Biolabs, leveraging advanced mass spectrometry platforms and expert analytical capabilities, provides high-quality and reliable bioactive peptides analysis services to accelerate your discovery of functional peptides and development of peptide-based therapeutics.

 

Feel free to contact us via Submit Inquiry or visit the MtoZ Biolabs website to obtain sample submission guidelines or request a customized project consultation. Let us help you unlock the full potential of every bioactive peptide candidate.

 

Related Services

Bioactive Peptide Component Identification and Quantification