Antibody Sequencing Service

High-quality, active, and effective antibody is a multi-billion-dollar industry. Merely having hybridoma cells is not enough due to potential risks such as storage accidents, genetic drift, or contamination. Therefore, performing sequence analysis on an antibody ensures that the antibody can be recombinantly produced at any time. Additionally, if you have obtained an effective antibody and wish to protect its intellectual property or expand its production, obtaining accurate antibody sequence is essential. Sequence analysis serves as the foundation for protecting your antibody and initiating antibody engineering projects, such as species, isotype, and subtype switching, or even antibody humanization.

 

MtoZ Biolabs’ MS-Based Monoclonal Antibody Sequence Analysis

When cell lines are lost or unavailable, sequencing hybridoma cells becomes infeasible. However, with advancements in MS technology, sufficient quantities of antibody samples can still be sequenced. Monoclonal antibodies (mAbs) are crucial in drug development and diagnostic reagent production. The primary structure of the antibody, especially the amino acid sequence of its CDR region, is central to its biological function. Accurate and rapid analysis of its complete sequence is critically important.

 

MtoZ Biolabs utilizes the world's highest resolution mass spectrometer, the Orbitrap Fusion Lumos, along with extensive bioinformatics expertise, establishing a next-generation antibody sequencing platform, which enables rapid and accurate analysis of the primary structure of antibodies. The platform is suitable for different antibody isotypes (e.g., IgG and IgM) and types (fluorescence-labeled antibodies, immobilized antibodies, antibodies from various species). MtoZ Biolabs' antibody sequencing service can analyze both the full-length and variable region amino acid sequences, providing clients with complete sequence information for the light and heavy chains. All sequencing services are performed independently in our laboratory, ensuring maximum protection of your intellectual property.

 

Service Advantages

1. Comprehensive Sequence Analysis

An optimized high-resolution mass spectrometry workflow and top-tier de novo sequencing software ensure thorough analysis of the entire antibody sequence from the N-terminal to the C-terminal.

 

2. High Accuracy

Each amino acid in the sequence is identified through signals from multiple peptide segments' MS/MS data.

 

3. Wide Applicability

This technology is applied to all antibody types (IgG, IgA, IgM, IgY, Fab, ScFv, etc.) and species (human, mouse, rat, rabbit, hamster, etc.). MtoZ Biolabs can also sequence conjugated and contaminated proteins and antibodies with two different light chains. Post-sequencing, the activity of murine and human antibodies is guaranteed.

 

4. Commitment to Excellence

MtoZ Biolabs’ antibody sequencing service is dedicated to MS-based sequence analysis. Our optimized workflow and bioinformatics analysis process ensure the highest quality service, even for the most challenging antibodies.

 

5. High Throughput

Whether sequencing a single antibody or hundreds, MtoZ Biolabs can meet your needs.

 

Services at MtoZ Biolabs

1. Sample Requirements

mAb sample amount > 50-100 μg. If your sample amount is < 50 μg, please contact us.

 

2. Turnaround Time

Typically around 3 weeks after receiving the sample.