4D Proteomics and Dual-Platform Full-Spectrum Metabolomics
- Cell or tissue samples: ≥ 50 mg (frozen storage)
- Cell lysates: ≥ 10 μg total protein
- Serum/plasma: ≥ 20 μL (avoid hemolysis)
- Cell or tissue samples: ≥ 20 mg (flash-frozen in liquid nitrogen)
- Serum/plasma: ≥ 100 μL (avoid hemolysis)
- Urine, feces, or other body fluids: ≥ 1 mL
- Metabolite standards: ≥ 10 μg
4D Proteomics and Dual-Platform Full-Spectrum Metabolomics is an advanced methodology that integrates 4D proteomics with dual-platform full-spectrum metabolomics, enabling a comprehensive analysis of dynamic biological changes. This approach provides in-depth molecular insights for precision medicine, drug discovery, and disease mechanism studies.
4D proteomics utilizes ion mobility mass spectrometry (IM-MS), incorporating ion mobility (IM) as a fourth dimension alongside conventional mass spectrometry parameters (m/z, retention time (RT), and intensity). This enhances peptide and protein separation efficiency and improves quantification accuracy, making it particularly suitable for low-abundance protein detection, post-translational modification (PTM) analysis, and protein interaction studies.
Dual-platform full-spectrum metabolomics combines liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) to achieve comprehensive coverage of both polar and nonpolar metabolites. LC-MS is optimized for the detection of amino acids, lipids, and nucleotides, while GC-MS is particularly suited for volatile organic acids, sugars, and fatty acids.
This integrated strategy enhances the holistic analysis of proteins and metabolites, providing deeper insights into biomarkers, metabolic pathways, and disease mechanisms.MtoZ Biolabs offers 4D Proteomics and Dual-Platform Full-Spectrum Metabolomics services that leverage advanced mass spectrometry instruments, including the Thermo Fisher Orbitrap Exploris 480 and timsTOF Pro 2, to provide high-precision, high-throughput analysis supporting cutting-edge research.
Analysis Workflow
MtoZ Biolabs follows a standardized workflow to ensure high data quality and reliability. Our 4D Proteomics and Dual-Platform Full-Spectrum Metabolomics approach utilizes advanced mass spectrometry to achieve comprehensive and precise proteomic and metabolomic profiling.
1. Sample Preparation and Quality Control
Optimized protein and metabolite extraction techniques enhance detection efficiency and ensure experimental reproducibility.
2. 4D Proteomics Data Acquisition
IM-MS-based peptide separation is performed, with high-resolution mass spectrometry improving proteomic data resolution and depth.
3. Dual-Platform Metabolomics Data Acquisition
LC-MS is used for detecting medium-polarity metabolites, while GC-MS is employed for volatile and low-polarity metabolites, ensuring comprehensive metabolic coverage.
4. Data Analysis and Integration
Multi-omics data integration enables pathway enrichment, metabolic regulation modeling, and protein-metabolite interaction analysis.
5. Results Delivery and Technical Support
A detailed analytical report is provided, including statistical analysis, functional annotation, and metabolic pathway interpretation, along with personalized technical support.
Service Advantages
· Multi-Dimensional Integrated Analysis: Combining 4D Proteomics and Dual-Platform Full-Spectrum Metabolomics enables deeper biological system analysis.
· High Sensitivity and Resolution: IM-MS enhances separation efficiency, improving protein and metabolite identification depth and quantification accuracy.
· Enhanced Time Resolution: 4D-DIA proteomics enables dynamic biological process studies, such as cellular metabolic adaptation and drug mechanism research.
· High Throughput and Data Integration: Suitable for large-scale sample analysis, supporting applications in systems biology and drug development.
· Customized Data Reports: Integrating machine learning algorithms and bioinformatics tools, this service provides tailored analysis strategies, facilitating innovative research breakthroughs.
Sample Submission Suggestions
To ensure high-quality and reproducible experimental data, MtoZ Biolabs provides detailed sample submission guidelines and recommends the following sample standards:
Proteomics Samples:
Metabolomics Samples:
For special sample preparation requirements or uncertainties regarding sample suitability, MtoZ Biolabs offers professional consultation and optimization strategies to ensure optimal experimental outcomes.
Case Study
Case 1: Identification of Potential Biomarkers in Placental Implantation Using 4D Proteomics and Dual-Platform Full-Spectrum Metabolomics
This study employed 4D label-free quantitative proteomics and untargeted metabolomics to analyze cervicovaginal fluid samples, identifying proteins and metabolites associated with placental implantation pathology. The study included six patients and six control pregnant women, screening key biomolecules such as arginine, GAL7, uPA, MMP9, and ITGAM. These molecules exhibited significant dysregulation in abnormal placental implantation, demonstrating their potential as early diagnostic and predictive biomarkers.
Jiang, H. et al. BMC Pregnancy Childbirth. 2024.
This case highlights the effectiveness and application potential of 4D Proteomics and Dual-Platform Full-Spectrum Metabolomics in biomarker research for disease studies.
FAQ
Q1: Why combine LC-MS and GC-MS in dual-platform metabolomics analysis?
LC-MS is suited for polar metabolites such as amino acids, lipids, and nucleotides, while GC-MS is ideal for low-polarity and volatile metabolites, including short-chain fatty acids and organic acids. MtoZ Biolabs employs a dual-platform strategy to ensure comprehensive metabolomic analysis.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs’ 4D Proteomics and Dual-Platform Full-Spectrum Metabolomics services integrate high-resolution ion mobility mass spectrometry (IM-MS) with LC-MS/GC-MS dual-platform metabolomics, providing high-throughput and precise protein-metabolite co-analysis. Whether for disease research, precision medicine, drug development, or systems biology, we offer professional solutions to drive groundbreaking scientific discoveries.
Contact us to receive personalized solutions—our team is ready to support your research with the highest level of expertise!
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