3-Hydroxybutyrylation Proteomics Analysis Service
3-Hydroxybutyrylation proteomics analysis is a high-throughput analytical technique specifically designed for studying protein 3-hydroxybutyrylation (Kbhb). 3-Hydroxybutyrylation is a novel post-translational modification (PTM) in which β-hydroxybutyrate (β-HB) covalently binds to lysine residues, influencing protein structure, function, and cellular metabolic regulation. This modification is closely associated with energy metabolism, ketogenesis, and epigenetic regulation, playing a crucial role in metabolic disorders, cancer, and neurodegenerative disease research. The technique primarily relies on high-resolution liquid chromatography-mass spectrometry (LC-MS/MS), combined with specific enrichment strategies, to achieve precise identification and quantification of 3-hydroxybutyrylation modification sites.
3-Hydroxybutyrylation proteomics analysis service is widely applied in metabolic disease studies, epigenetics, cancer research, and aging mechanism exploration. For instance, in diabetes and obesity research, this modification serves as a key regulatory signal in energy metabolism; in cancer research, 3-hydroxybutyrylation may influence cell proliferation and metabolic adaptability, making it a potential therapeutic target. Additionally, this modification plays a significant role in epigenetics by regulating histone modifications, thereby exerting a profound impact on gene expression regulation. This service provides a precise analytical tool for elucidating the biological functions, disease mechanisms, and drug targets of 3-hydroxybutyrylation.
Li, Z H. et al. Biomacromolecules, 2021.
Figure 1. Lysine 3-Hydroxybutyrylation.
Services at MtoZ Biolabs
Based on Thermo Fisher's Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometry platforms, in combination with Nano-LC, MtoZ Biolabs provides advanced 3-hydroxybutyrylation proteomics analysis service that enables precise identification and quantification of modified proteins. This service encompasses sample preparation, specific enrichment, high-throughput mass spectrometry detection, and bioinformatics analysis, ensuring high sensitivity and high-resolution data acquisition.
Service Advantages
1. High Sensitivity and High Resolution
MtoZ Biolabs utilizes high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology to establish the 3-hydroxybutyrylation proteomics analysis service platform, ensuring the precise identification of 3-hydroxybutyrylation modifications. This platform enables the detection of low-abundance modified proteins and enhances overall detection coverage.
2. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all 3-hydroxybutyrylation proteomics analysis data providing clients with acomprehensive data report.
4. Customized Services
MtoZ Biolabs offers flexible experimental design and personalized data analysis tailored to client needs, supporting scientific research in metabolic diseases, epigenetics, cancer studies, and new drug development.
Sample Submission Suggestions
Notice: 1. If you are providing tissue samples, please ship them to us on dry ice.
2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.
3. If you have special requirements or need assistance with sample preparation, please contact us.
Applications
1. Metabolic Disease Research
3-Hydroxybutyrylation plays a crucial role in diabetes, obesity, and ketone metabolism. The 3-hydroxybutyrylation proteomics analysis service can be utilized to investigate the molecular mechanisms of energy metabolism disorders, identify key modified proteins, and explore potential therapeutic targets.
2. Epigenetic Regulation
3-Hydroxybutyrylation modifications influence histone modification states and gene expression. This service enables the study of its regulatory mechanisms in epigenetics, uncovering novel gene regulation patterns.
3. Cancer Research
Metabolic reprogramming in cancer cells involves 3-hydroxybutyrylation modifications. The 3-hydroxybutyrylation proteomics analysis service can help analyze tumor cell metabolic adaptability and modification-related signaling pathways, aiding targeted cancer therapy research.
4. Aging and Neurodegenerative Diseases
Studies have shown that 3-hydroxybutyrylation is associated with aging and neurodegenerative diseases such as Alzheimer's and Parkinson's. This service enables the analysis of metabolic changes and protein modification patterns in neural cells, providing insights into disease pathogenesis.
5. Drug Discovery and Biomarker Screening
3-Hydroxybutyrylation modifications may serve as biomarkers for metabolism-related diseases. The 3-hydroxybutyrylation proteomics analysis service can be utilized for drug target screening and mechanism-of-action studies, supporting precision medicine development.
Case Study
1. Ketogenesis Impact on Liver Metabolism Revealed by Proteomics of Lysine β-Hydroxybutyrylation
This study aims to investigate the impact of ketogenesis on liver metabolism and systematically analyze the modification patterns and biological functions of lysine β-hydroxybutyrylation (Kbhb) in the liver using proteomic approaches. The study subjects were mouse liver tissues, where ketogenesis was induced through fasting and high-fat diets to elevate β-hydroxybutyrate (BHB) levels. High-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with a specific antibody enrichment strategy was employed to comprehensively identify and quantify β-hydroxybutyrylation modifications in hepatic proteins. The results demonstrated that β-hydroxybutyrylation was significantly upregulated under ketogenesis, predominantly enriched in key metabolic pathways such as glycolysis, fatty acid oxidation, and the TCA cycle, and closely associated with enzymatic activity changes. Furthermore, functional analysis suggested that this modification may facilitate the liver’s adaptation to ketogenesis by regulating the activity and stability of metabolic enzymes. The study concludes that β-hydroxybutyrylation is a crucial post-translational modification involved in liver metabolic regulation, providing new insights into ketone metabolism regulation and potential targets for metabolic disease interventions.
Koronowski, K B. et al. Cell Reports, 2021.
Figure 3. Characterizing the Lysine β-Hydroxybutyrylome in Mouse Liver.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. The Detailed Information of 3-Hydroxybutyrylation Proteomics Analysis
4. Mass Spectrometry Image
5. Bioinformatics Analysis
6. Raw Data
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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