Will the Protein Sample Degrade if Left at Room Temperature Overnight

Protein stability is primarily influenced by the specific protein, sample composition, and storage conditions. However, for most proteins, storage at room temperature overnight can result in degradation.

 

For critical protein samples, freezing immediately following purification and storing at -80°C is advisable. Alternatively, adding suitable stabilizers or protease inhibitors and storing at 4°C for short-term preservation can help maintain stability. For long-term storage, samples should be kept at -20°C or -80°C.

 

To determine whether protein degradation has occurred, its integrity can be assessed using SDS-PAGE. The presence of unexpected smaller bands alongside the primary protein band may indicate degradation products.

 

MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

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