Why Are the Marker Bands Curved in SDS-PAGE While Protein Bands Appear Normal
If the marker (also referred to as molecular weight standard or molecular weight ladder) exhibits a curved or hook-like appearance during SDS-PAGE electrophoresis, while the protein bands remain unaffected, several factors may be responsible:
1. Issues with the Electrophoresis Apparatus
Poor contact between electrodes or an unstable power supply can result in uneven current distribution, which may interfere with the migration of the marker. In such cases, the electrophoresis system and power source should be thoroughly inspected to ensure proper functioning.
2. Gel-Related Inconsistencies
Uneven gel polymerization or mechanical damage to the gel during sample loading can also disrupt the marker's migration. Careful attention must be paid to both gel preparation and the sample loading process to ensure they are executed under standardized and controlled conditions.
3. Compromised Marker Quality
Improper preparation or storage conditions of the marker may alter its physicochemical properties, adversely affecting its electrophoretic behavior. It is essential to verify that the marker was prepared and stored under recommended conditions.
4. Excessive Current or Voltage
Applying overly high current or voltage may lead to distortion of the bands. Optimizing electrophoretic parameters is necessary to avoid artifacts associated with excessive electrical input.
A systematic examination of the above potential causes will help identify the source of the problem, allowing for appropriate corrective actions to be taken.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
