Which Online Tools Can Compare Antibody Heavy and Light Chain Variable Region Sequences?
Several specialized bioinformatics tools and databases can be used to align and compare the variable region sequences of antibody heavy and light chains. These platforms not only facilitate sequence alignment but also provide structural and functional insights into antibodies. Below are commonly used tools:
IMGT (International ImMunoGeneTics Information System)
1. Description
IMGT/V-QUEST is a dedicated tool for immunoglobulin (Ig) and T cell receptor (TCR) sequence analysis. It allows alignment of antibody heavy and light chain variable region sequences, identifies V(D)J gene segments, and performs mutation analysis.
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NCBI IgBLAST
1. Description
A BLAST-based tool specialized for immunoglobulin and TCR sequences. It enables comparison of antibody variable region sequences and identifies corresponding V, D, and J gene segments.
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Abysis
1. Description
An antibody sequence database and analysis platform offering sequence alignment, annotation, and structural prediction. Users can upload antibody sequences for comparison with curated database entries.
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ANARCI
1. Description
A tool for antibody and TCR sequence annotation, which assigns numbering based on the IMGT, Kabat, and Chothia schemes. It enables rapid identification and annotation of variable region sequences.
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Clustal Omega
1. Description
A general-purpose multiple sequence alignment tool that can be used for comparing antibody sequences. While not specifically designed for antibody analysis, it remains a useful option for aligning multiple antibody sequences.
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MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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