Which Amino Acid Serves as the Attachment Site for the Oligosaccharide Chain in N-Linked Glycosylation?
In N-glycosylation, the oligosaccharide chain is typically covalently linked to the amide nitrogen of the side chain of an asparagine residue located within a specific amino acid sequence motif, NXS/T (where X represents any amino acid except proline), which is recognized by oligosaccharyltransferase. This glycosylation event constitutes an integral step in intracellular biosynthesis and plays a pivotal role in protein stability, correct folding, and biological function.
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