What Sugar Appears When Mannose and Rhamnose Co-elute in HPLC Monosaccharide Analysis
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On amino columns (NH₂ columns), mannuronic acid typically elutes within 10–20 minutes.
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On reversed-phase columns (C18 columns), when a buffered mobile phase is employed, the retention time may also fall within or exceed the 10–20 minute range.
Elution Behavior of Mannuronic Acid
Mannuronic acid is an acidic monosaccharide that typically exhibits a longer retention time than neutral monosaccharides. Under standard HPLC conditions, it generally elutes after mannose and rhamnose. However, its exact retention time can vary depending on parameters such as column type, mobile phase composition, and pH. Specifically:
Potential Compounds Eluting Between Mannose and Rhamnose
Compounds eluting between mannose and rhamnose may include other monosaccharides or structurally related sugar analogs. Common examples include:
1. Glucose
A ubiquitous monosaccharide, glucose often elutes between mannose and rhamnose under typical chromatographic conditions.
2. Galactose
Structurally similar to mannose, galactose tends to exhibit a slightly longer retention time than mannose.
3. Fructose
As a ketose, fructose commonly elutes in the same region between mannose and rhamnose.
4. Xylose
A five-carbon sugar (pentose), xylose may also elute in the retention window between mannose and rhamnose.
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