What Software Is Used for Glycosylation Site Prediction? How to Interpret the Results
Glycosylation site prediction is typically carried out using software tools such as NetNGlyc, GlycoEP, and NetOGlyc. These programs are designed to identify both N-linked and O-linked glycosylation sites within protein sequences. To use these tools, users input the protein sequence of interest, after which the software analyzes the sequence to identify potential glycosylation sites. Each identified site is accompanied by a prediction score or probability value, with higher scores generally indicating a greater likelihood of glycosylation.
The prediction results are commonly presented as tables or graphical outputs, detailing the position of each amino acid in the sequence, the likelihood of it being a glycosylation site, and the corresponding prediction score. For N-linked glycosylation, the analysis typically targets the canonical Asn-X-Ser/Thr motif (where X represents any amino acid except proline). In contrast, O-linked glycosylation site prediction relies more heavily on algorithm-based pattern recognition rather than fixed sequence motifs.
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