What Reagents and Their Names Are Required in Metabolomics?
In metabolomics, the reagents commonly employed are typically associated with sample preparation, metabolite extraction, derivatization, and analysis. Below are some widely used reagents at each stage of metabolomics research:
Sample Preparation and Extraction
1. Methanol
Frequently used in sample pretreatment and extraction.
2. Acetonitrile
Utilized for metabolite extraction and protein precipitation.
3. Chloroform
Commonly employed for extracting lipid metabolites.
Derivatization Reagents
1. Formamide
Used in the derivatization of amino acids and other metabolites.
2. Benzylamine chloride
Applied in the derivatization of phenolic compounds.
Internal Standards
1. D4-Cyclodextrin
Used as an internal standard for normalization and calibration in metabolomics experiments.
2. 13C-Glucose
Serves as an internal standard for carbon metabolism studies.
Analytical Reagents
1. Buffers
Common buffers such as phosphate and acetate buffers are used in liquid chromatography.
2. Mass Spectrometry Calibration Solutions
These are critical for the calibration of mass spectrometers, ensuring accurate and reproducible results.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
