Western Blot Quantification Service
- High-Precision Quantification: Advanced image analysis ensures accurate signal measurement and normalization.
- Expert Team: Experienced technicians maintain standardized and reproducible workflows.
- Premium Antibody Resources: High-specificity antibodies enhance detection sensitivity.
- Tailored Solutions: Customized plans for specific protein detection and data analysis.
- One-Time-Charge:Our pricing is transparent, no hidden fees or additional costs.
- Disease Biomarkers: Quantify protein levels in cancer, metabolic disorders, and neurodegenerative diseases.
- Signaling Pathways: Monitor changes in key signaling proteins under various conditions.
- Drug Development: Evaluate drug effects on target proteins.
- Post-Translational Modifications: Analyze modifications like phosphorylation or acetylation.
- Gene Expression: Validate gene expression at transcriptional and translational levels.
Western blot quantification is a critical approach for accurately measuring protein expression levels and assessing functional changes. While it is most commonly used for quantifying proteins, this technique can also be adapted for the detection and quantification of other biomolecules, such as nucleic acids and small molecules, under specific conditions. By quantifying these substances, researchers can gain valuable insights into cellular signaling pathways, disease mechanisms, and drug effects. At MtoZ Biolabs we offer a precise and reliable western blot quantification service that leverages cutting-edge platforms and specialized software to deliver high-accuracy results, empowering researchers to achieve impactful scientific discoveries and meet their research goals.
Analysis Workflow
The western blot quantification service follows a meticulous and scientifically rigorous workflow, ensuring precise and reliable data for clients. The workflow includes:
1. Total Protein Extraction and Quantification
Sample Sources: Including tissues, cells, and serum. Total protein is extracted efficiently using optimized lysis buffers to minimize degradation.
Protein Quantification: Accurate quantification using BCA or Bradford assays ensures consistent sample loading for subsequent analyses.
2. SDS-PAGE Separation
Gel Preparation: High-resolution polyacrylamide gels with optimized conditions are used to separate proteins based on molecular weight.
Electrophoresis: Proteins are separated under controlled voltage conditions, ensuring clear, well-defined bands for target proteins.
3. Protein Transfer (Blotting)
Materials: Nitrocellulose (NC) or polyvinylidene difluoride (PVDF) membranes are chosen based on protein abundance.
Transfer Techniques: Wet and semi-dry transfer methods are employed to maximize protein retention on membranes.
4. Membrane Blocking and Antibody Incubation
Blocking: Non-specific sites are blocked using BSA or skim milk to reduce background noise.
Antibody Incubation: High-specificity primary and labeled secondary antibodies are applied for optimal detection.
5. Protein Detection and Signal Capture
Detection Methods: Chemiluminescence (ECL) for low-abundance proteins or fluorescent scanning for multiplex detection.
Data Imaging: High-resolution CCD systems ensure reproducibility and accuracy.
6. Quantitative Analysis and Reporting
Analysis: Band intensity is quantified using software like ImageJ, normalizing signals to compare protein expression.
Reporting: Comprehensive reports include images, quantitative data, and experimental conditions.
Meftahi, G. H. et al. Biochem Mol Biol Educ. 2021.
Service Advantages
At MtoZ Biolabs, we provide high-precision western blot quantification service designed to meet the most demanding research requirements. Our advanced image analysis tools and expert team ensure reliable and reproducible results, whether you’re conducting basic research or validating complex biomarkers.
Sample Submission Suggestions
1. Sample Types
Serum, plasma, urine, tissue extracts, and other biological samples.
2. Sample Volume
At least 200 μL of liquid sample or 200 mg of solid sample.
3. Sample Preservation
Store samples at -20°C or lower to prevent degradation, avoiding repeated freeze-thaw cycles.
Note: Provide details on sample collection and handling.
Applications
Our western blot quantification service supports diverse research areas, enabling you to gain deeper insights into protein dynamics and regulatory mechanisms:
FAQ
Q1: How to verify the quantitative reliability of western blot quantification results?
The semi-quantitative reliability of western blot depends on multiple factors, including sample loading, selection of internal control proteins, and the linear range of signal detection. MtoZ Biolabs adopts a strict quality control system, ensuring high consistency of results through internal control calibration and repeat experiments. Equipped with advanced signal detection platforms (e.g., chemiluminescence and fluorescence systems) and performing multiple repeat experiments, we control quantitative errors within 5%, helping clients obtain highly reliable quantitative data.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Western Blot Imaging and Quality Control Assessment
4. Data Analysis and Quantification
5. Bioinformatics and Statistical Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our western blot quantification service is designed to provide high-throughput, cost-effective solutions with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.
How to order?
